权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

IN VITRO CULTURE MODEL FOR HEPATITIS C VIRUS GROWTH

丙型肝炎病毒生长的体外培养模型

基本信息

批准号：
2886077
负责人：
REGINO P GONZALEZ-PERALTA
金额：
$ 8.96万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-01 至 2000-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2886077
关键词：
biological models hepatitis C virus liver cells low density lipoprotein receptor microorganism culture model design /development polymerase chain reaction tissue /cell culture virus replication

项目摘要

DESCRIPTION: The candidate obtained his M.D. degree from the Universidad Central del Este (location not specified), followed by a Pediatric internship and residency at the Cook County Hospital in Chicago, and then a Pediatric Gastroenterology fellowship training at his current institution, the University of Florida at Gainesville, where he was promoted to an Assistant Professor in 1994 after three years of fellowship and one year of serving as a chief Pediatric resident. The candidate proposes to obtain research training working in his own space within the "pediatric core laboratory" but under the supervision of Dr. Lau, who is an Associate Professor in the Department of Medicine at the University of Florida at Gainsville. The candidate presents data regarding a cell culture model supporting HCV replication. He proposes to optimize in vitro HCV replication in this liver-derived cell culture model by manipulating the inoculation conditions, using inducers of cell differentiation, and stimulating LDL receptor expression. He plans to use specific assays for the detection and quantitation of HCV replication in the cell culture model. He further proposes to use this model to study HCV quasispecies. Training Plan and Goals. Dr. Lau (the proposed mentor) plans to continue his supervision of the candidate. However, it is not clear what this supervision entails. There is no plan outlined in the application for formal didactic training of the candidate. Participating Faculty. The mentor, Dr. Johnson Lau, is a well known investigator in this field but his expertise is not in the area of cell biology. He has a long list of trainees most of whom were/are fellows who have been successful in getting their work published. However, it is not clear if any of the trainees have gone on to become independent investigators with their own funding. Fellow. The applicant, Dr. R. Gonzalez-Peralta, has some experience in hepatitis C research as evidenced by the previous publications. He has completed his fellowship training and has been an Assistant Professor in the Department of Pediatrics since 1994. Resources and Environment. The resources are adequate and the Department of Pediatrics appears committed to provide the protected time necessary for the applicant. The applicant and his mentor's laboratories are well equipped to perform the proposed work.

描述：候选人获得了医学博士学位。大学学位 Central del Este（未指定位置），然后是儿科在芝加哥的库克县医院实习和住院，然后在他目前所在的机构接受儿科胃肠病学奖学金培训，在盖恩斯维尔的佛罗里达大学，他被晋升为 1994年，在获得三年研究金和一年担任儿科住院总医师候选人建议获得研究培训工作在他自己的空间内的“儿科核心” 实验室”，但在刘博士的监督下，谁是助理佛罗里达大学医学系教授，盖恩斯维尔候选人介绍了关于支持HCV的细胞培养模型的数据复制的他建议优化体外HCV复制，肝源性细胞培养模型，使用细胞分化诱导剂，刺激LDL受体，表情他计划使用特定的检测方法进行检测，细胞培养模型中HCV复制的定量。他进一步建议使用该模型来研究HCV准种。培训计划和目标。刘博士（拟议的导师）计划继续他对候选人的监督。然而，目前尚不清楚这是什么。监督需要。申请书中没有概述以下计划：对候选人进行正式的教学培训。参与教师。导师约翰逊刘博士是一位著名的他是这一领域的调查员，但他的专长不是细胞领域。生物学他有一长串受训者名单，其中大多数是研究员，成功地发表了他们的作品。但不很清楚是否有任何受训者已经独立研究人员用自己的资金。家伙. 申请人R博士冈萨雷斯-佩拉尔塔，有一些经验，丙型肝炎研究，如以前的出版物所证明的。他完成了他的奖学金培训，并一直在助理教授自1994年以来，儿科。资源与环境。资源充足，儿科似乎致力于提供必要的保护时间，申请人。申请人及其导师的实验室设备齐全，执行拟议的工作。