IN VITRO CULTURE MODEL FOR HEPATITIS C VIRUS GROWTH

描述：这位候选人在该大学获得医学博士学位 埃斯特中心(位置未指明)，后面是儿科 在芝加哥的库克县医院实习和实习，然后 在他目前的机构接受了儿科胃肠病奖学金培训， 在盖恩斯维尔的佛罗里达大学，他在那里被提升为 在经历了三年的研究员生涯和一年的 担任儿科总住院医师。候选人提议获得 在自己的研究培训空间内工作的“儿科核心” 化验室“，但由刘医生监督，刘博士是一名助理医生 佛罗里达大学医学系教授 盖恩斯维尔。 候选人提交了关于支持丙型肝炎病毒的细胞培养模型的数据 复制。他建议优化丙型肝炎病毒的体外复制 通过控制接种条件建立肝源性细胞培养模型， 使用细胞分化诱导剂，刺激低密度脂蛋白受体 表情。他计划使用特定的检测方法来检测和 细胞培养模型中丙型肝炎病毒复制的定量。他进一步 建议使用该模型来研究丙型肝炎病毒准种。 培训计划和目标。刘博士(建议的导师)计划继续 他对候选人的监督。然而，目前还不清楚这是什么 监督是必要的。申请表中没有列出任何计划 对应聘者进行正式的教育培训。 参赛学院。导师刘强森博士是一位知名的 这一领域的调查员，但他的专业知识不在细胞领域 生物学。他有一长串的实习生名单，其中大多数都是 已经成功地出版了他们的作品。然而，它并不是 清楚是否有实习生后来独立了 调查人员用他们自己的资金。 伙计。申请人R.Gonzalez-Peralta博士在以下方面有一定经验 先前的出版物证明了丙型肝炎的研究。他有 完成了他的研究员培训，并一直担任 儿科自1994年起就诊。 资源与环境。资源充足，该部 儿科似乎致力于提供必要的保护时间 申请人。申请人和他导师的实验室设备齐全，可以 执行建议的工作。

DESCRIPTION: The candidate obtained his M.D. degree from the Universidad Central del Este (location not specified), followed by a Pediatric internship and residency at the Cook County Hospital in Chicago, and then a Pediatric Gastroenterology fellowship training at his current institution, the University of Florida at Gainesville, where he was promoted to an Assistant Professor in 1994 after three years of fellowship and one year of serving as a chief Pediatric resident. The candidate proposes to obtain research training working in his own space within the "pediatric core laboratory" but under the supervision of Dr. Lau, who is an Associate Professor in the Department of Medicine at the University of Florida at Gainsville. The candidate presents data regarding a cell culture model supporting HCV replication. He proposes to optimize in vitro HCV replication in this liver-derived cell culture model by manipulating the inoculation conditions, using inducers of cell differentiation, and stimulating LDL receptor expression. He plans to use specific assays for the detection and quantitation of HCV replication in the cell culture model. He further proposes to use this model to study HCV quasispecies. Training Plan and Goals. Dr. Lau (the proposed mentor) plans to continue his supervision of the candidate. However, it is not clear what this supervision entails. There is no plan outlined in the application for formal didactic training of the candidate. Participating Faculty. The mentor, Dr. Johnson Lau, is a well known investigator in this field but his expertise is not in the area of cell biology. He has a long list of trainees most of whom were/are fellows who have been successful in getting their work published. However, it is not clear if any of the trainees have gone on to become independent investigators with their own funding. Fellow. The applicant, Dr. R. Gonzalez-Peralta, has some experience in hepatitis C research as evidenced by the previous publications. He has completed his fellowship training and has been an Assistant Professor in the Department of Pediatrics since 1994. Resources and Environment. The resources are adequate and the Department of Pediatrics appears committed to provide the protected time necessary for the applicant. The applicant and his mentor's laboratories are well equipped to perform the proposed work.