DIETARY EFFECTS ON MEMBRANE LIPIDS

饮食对膜脂的影响

• 批准号: 2836714
• 负责人: Tim Arden Lyerla
• 金额: $ 10.33万
• 依托单位: CLARK UNIVERSITY (WORCESTER, MA)
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2836714
• 关键词: Chediak Higashi syndrome; dietary lipid; enzymes; laboratory mouse; lipid metabolism; lysosomes; male; membrane lipids; mutant; nutrition related tag; omega 3 fatty acid; phosphatidylcholines; secretion; urinalysis

DESCRIPTION (adapted from the applicant's abstract): The long-range goal of this proposal is to understand the role of membrane lipids in the formation and utilization of membrane organelles. Control male mice secrete lysosomal enzymes and multilamellar bodies from the renal proximal tubule cells into the urine. These bodies are enriched in phosphatidylcholine containing C22:6-n-3 fatty acid that must be derived from the diet. The secretion of multilamellar bodies and lysosomal enzymes is blocked in human Chediak-Higashi syndrome and in the beige mouse mutant. This led to a working hypothesis that this lipid plays some role in the secretory process leading to urinary excretions of these lysosomal materials. The specific aims of this proposal are to investigate the influence of dietary lipids (docosahexaenoic acid, C22:6n-3) on the formation of lysosomal-like multilamellar bodies in control and Chediak-Higashi CHS) mice. The investigator proposes to (i) Determine if n-3 fatty acid levels in the diet affect urinary secretion of lysosomal enzymes and multilamellar bodies in control male mice, and (ii) Determine if n-3 fatty acid levels in the diet affect the block to urinary secretion in beige mutant male mice.

描述（改编自申请人的摘要）： 该建议是为了了解膜脂质在形成和 膜细胞器的利用。对照组雄性小鼠分泌溶酶体酶 和多板层体从肾近端小管细胞进入尿液。 这些身体富含含有C22：6-n-3脂肪的磷脂酰胆碱 必须从饮食中获得的酸。多层小体的分泌 和溶酶体酶在人Chediak-Higashi综合征中被阻断， 米色老鼠突变体。这导致了一个工作假设，这种脂质发挥了一些作用， 在导致这些溶酶体的尿排泄的分泌过程中的作用 材料.本提案的具体目的是调查影响 膳食脂质（二十二碳六烯酸，C22：6 n-3）对形成 对照和Chediak-Higashi CHS）小鼠中的溶酶体样多层体。 研究者建议（i）确定饮食中的n-3脂肪酸水平是否 影响尿中溶酶体酶和多层小体的分泌 对照雄性小鼠，和（ii）确定饮食中的n-3脂肪酸水平 影响米色突变雄性小鼠的尿分泌阻滞。