REGULATION OF METABOLISM BY HYPOXIA AND OSMOTIC PRESSURE

通过缺氧和渗透压调节代谢

• 批准号: 3023441
• 负责人: Jacob J Blum
• 金额: $ 3.15万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-29 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3023441
• 关键词: Leishmania; cell osmotic pressure; glucose metabolism; hypoxia; microorganism metabolism; tissue /cell culture

In recent years, much has been learned about the structural organization and regulation of the pathways of intermediary metabolism in the promastigote form of leishmania which lives in the intestinal tract of phlebotomine flies. Very little is known about the metabolism of the amastigote forms that live in the phagolysosome of human macrophages and cause leishmaniasis. It is now possible to grow the amastigote form of Leishmania mexicana pifanoi in axenic culture, and this opens the way to understanding its metabolism and to study the sequential changes that occur during differentiation from one form to the other. These studies will become an integral part of my research program at Duke University Medical Center and will lead to an ongoing collaboration between Dr. Opperdoes and myself.

近年来，人们对结构组织有了很多了解， 和调节中间代谢的途径， 利什曼原虫的前鞭毛体形式，生活在肠道中， 白蛉。 对人体的新陈代谢知之甚少， 无鞭毛体形式，生活在人类巨噬细胞的吞噬溶酶体中， 引起利什曼病。 现在有可能培养出无鞭毛体形式的 墨西哥利什曼原虫pifanoi在无菌培养，这开辟了道路， 了解它的新陈代谢，研究它的连续变化， 从一种形式到另一种形式的分化。 这些 我的研究将成为我在杜克大学研究计划的一个组成部分 大学医学中心，并将导致正在进行的合作 我和奥珀多斯医生之间的关系

项目成果

Effects of hypoxia, acute osmotic stress, and protein kinase inhibitors on the intermediary metabolism of Leishmania.

缺氧、急性渗透应激和蛋白激酶抑制剂对利什曼原虫中间代谢的影响。

• DOI: 10.1111/j.1550-7408.1994.tb05925.x
• 发表时间: 1994
• 期刊: The Journal of eukaryotic microbiology
• 作者: Blum,JJ