NEUROENDOCRINOLOGY OF SALT APPETITE AND BLOOD PRESSURE

这份提案是对ADAMHA研究科学家的申请 发展奖(一级)。有监督的培训和培训计划 建议进行研究，候选人将通过该研究进行研究 神经内分泌底物的遗传和环境来源 动态平衡行为和生理过程中的变化。 继续教授定量放射自显影图像分析和 分子生物学方法方面的广泛培训将由 培训顾问，并辅之以讲习班和课程。作为该计划的一部分 建议的培训和研究，候选人将调查 脑醛固酮结合与脑血管紧张素原基因的关系 表达对模型激励行为(食盐消费)的控制 和一个调节的生理过程(循环)。遗传 对这些过程的影响将通过分析 行为、生理和神经内分泌学的表型变异 在选择纯合子的品系间和品系内育种之后 具有良好行为和生理表型特征的大鼠。 环境对食盐食欲、循环、大脑的影响 将评估醛固酮结合和血管紧张素原基因的表达 在钠缺乏挑战后，或母亲行为中断后，或 断奶前期的钠平衡。心理健康的相关性 拟议的研究源于它将为 遗传和环境对动态平衡行为的影响及其可能 这些影响是通过神经内分泌机制进行调节的。 拟议工作中提供的培训将为应聘者提供 重要的分子神经科学技术将会有广泛的应用 在未来的心理生物学研究中的适用性。

This proposal is an application for an ADAMHA Research Scientist Development Award (Level I). A program of supervised training and research is proposed, through which the candidate will study neuroendocrine substrates of genetic and environmental sources of variance in homeostatic behavioral and physiological processes. Continued instruction in quantitative autoradiographic image analysis and extensive training in molecular biological methods will be provided by Advisors, and supplemented by workshops and courses. As part of the proposed training and research, the Candidate will investigate the relationship of brain aldosterone binding and brain angiotensinogen gene expression to control of a model motivated behavior (salt consumption) and a regulated physiological process (the circulation). Genetic influences on these processes will be studied through analysis of phenotypic variation in behavior, physiology and neuroendocrinology subsequent to selective inter-and intra-strain breeding of homozygous rats with well characterized behavioral and physiological phenotypes. Environmental influences on salt appetite, the circulation, brain aldosterone binding and angiotensinogen gene expression will be evaluated after sodium depletion challenges, or disruption of maternal behavior or sodium balance in the preweaning period. The mental health relevance of the proposed research derives from the insights that it will provide into genetic and environmental influences on homeostatic behavior and possible neuroendocrine mechanisms through which these influences are mediated. The training provided in the proposed work will provide the Candidate with important molecular neuroscience techniques that will have broad applicability in future psychobiological research.