CYTOTOXIC LYMPHOCYTES: IMMUNE FUNCTION & ITS REGULATION

细胞毒性T淋巴细胞（CTL）在移植排斥反应中起重要作用 和抗病毒保护。 此外，这些细胞提供了一个独特的模型， 用于研究细胞-细胞相互作用和诱导细胞功能。 当CTL或其祖细胞遇到在其表面表达 CTL具有受体的抗原，许多结果可以 随后：1）不成熟或记忆CTL可以启动一系列事件 导致其功能成熟; 2）完全分化的CTL可以 启动靶细胞裂解; 3）成熟和未成熟细胞都可以启动 导致细胞分裂的一系列事件; 4）成熟细胞（未成熟的 细胞？）释放淋巴因子 我们已经开发了克隆模型，这将使我们开始解剖 这些功能反应的组成部分。 再加上我们的 理解这些不同功能组件的性质将是 研究表征调节其表达的免疫信号。 最后，我们将开始一系列实验， 我们克隆体的独特特征可以让我们选择 一系列功能机制有缺陷的突变CTL。 使用 在标准的体细胞遗传实验中， 让我们开始讨论个体之间的互动 将受体结合的信号传递到受体所需的成分 生物功能的表达。

The cytotoxic T lymphocyte (CTL) plays an important role in graft rejection and antiviral protection. In addition, these cells provide a unique model for studies of cell-cell interaction and induction of cellular function. When the CTL or its progenitor encounters a cell expressing on its surface an antigen for which the CTL has a receptor, a number of consequences can ensue: 1) an immature or memory CTL can initiate a series of events leading to its functional maturation; 2) a fully differentiated CTL can initiate target cell lysis; 3) both mature and immature cells can initiate the series of events leading to cell division; 4) mature cells (immature cells?) release lymphokines. We have developed clonal models that will allow us to begin to dissect the components of each of these functional responses. Coupled to our understanding of the nature of these various functional components will be studies characterizing the immune signals regulating their expression. Finally, we will begin a series of experiments designed to take advantage of unique characteristics of our clones that may allow us to select for a series of mutant CTL that have defects in their functional machinery. Use of these mutant cells in standard somatic cell genetic experiments will allow us to begin to address the interaction between the individual components required to transmit the signal from receptor binding to the expression of biological function.