LAMININ AND FIBRONECTIN

该 RCDA 提案的长期研究目标是了解 细胞外基质（ECM）分子在周围神经中的作用 系统（PNS）开发。 ECM 分子调节细胞形状、运动和 胚胎发生过程中的分化和粘附糖蛋白 纤连蛋白 (FN) 和层粘连蛋白 (LAM) 在空间和时间上定位 介导神经嵴分化和神经纤维的各个方面 伸长率。三个具体目标定义了解决这个问题的方法。 首先，FN 和 LAM 的细胞结合域与 细胞与完整分子的相互作用？组织培养研究将 探针 1) 粘附在细胞与每个结构域相互作用中的作用，2) 活细胞与特定域相互作用时的行为，以及 3) 这些相互作用的细胞骨架相关性。二、FN和LAM怎么样 被发育中的三七总皂甙中的细胞所利用？方法将是 光和电子显微镜水平的免疫细胞化学， 分析细胞与体外复杂基质的相互作用，以及 体内细胞-ECM 相互作用的扰动。三、角色有哪些 FN 和 LAM 的定义区域在迁移和分化中的作用 神经嵴细胞？ 在 RCDA 的资助下，P.I.将扩展至神经嵴的方法 差异化，但学到的技术将适用于研究 以及神经元行为。较长的时间将花费在 10 伦敦国王学院 Jeremey Brockes 博士的实验室进行研究 重组DNA方法分化亚群 胚胎和再生细胞，以及 2) 神经科学研究所 大学。俄勒冈州（尤金）的几个实验室正在研究神经 使用多种技术（例如，斑马鱼系统开发） 分子生物学，活体动物细胞激光消融，单克隆 抗体产生）。 随着教学和委员会职责的释放，P.I. 也将能够更有效地寻求研究机会 大学。新墨西哥州。尤其是卓越的图像分析 新墨西哥大学的设施将得到利用。这次也将允许 P.I. 开发发育神经生物学研究生课程 吸引学生参与该领域研究的意图。

The long-range research goal of this RCDA proposal is to understand the roles of extracellular matrix (ECM) molecules in peripheral nervous system (PNS) development. ECM molecules regulate cell shape, movement and differentiation during embryogenesis, and the adhesive glycoproteins fibronectin (FN) and laminin (LAM) are located spatially and temporally to mediate aspects of neural crest differentiation and nerve fiber elongation. Three Specific Aims define approaches to this question. First, what is the relevance of the cell binding domains of FN and LAM to cell interaction with the intact molecule? Tissue culture studies will probe 1) roles of adhesion in cell interaction with each domain, 2) behavior of living cells as they interact with specific domains, and 3) cytoskeletal correlates of these interactions. Second, how are FN and LAM used by cells in the developing PNS? Approaches will be immunocytochemistry at the light and electron microscopic levels, analysis of cell interaction with complex matrices in vitro, and perturbation of cell-ECM interactions in vivo. Third, what are the roles of defined regions of FN and LAM in migration and differentiation of neural crest cells? With RCDA funding, the P.I. will extend approaches to neural crest differentiation, but the techniques learned will be applicable to studies of neuronal behavior as well. Extended periods of time will be spent in 10 the lab of Dr. Jeremey Brockes, Kings College London, to study recombinant DNA approaches to differentiation of subpopulations of embryonic and regenerating cells, and 2) the Neuroscience Institute at the Univ. of Oregon (Eugene) where several labs are studying nervous system development in zebrafish using a wide range of techniques (eg. molecular biology, lasar ablation of cells in living animals, monoclonal antibody production). With time release from teaching and committee responsibilities, the P.I. also will be able to pursue more effectively research opportunities at the Univ. of New Mexico. In particular, the exceptional image analysis facilities at UNM will be exploited. This time will also permit the P.I. to develop a graduate course in developmental neurobiology, with the intention of attracting students to this area of research.