GENES ENCODING HUMAN B CELL DIFFERENTIATION ANTIGENS

表达在细胞膜上的糖蛋白库 淋巴样细胞反映了它们的分化和成熟状态。 这些分子中的许多是使用单克隆抗体首次鉴定的 对抗肿瘤细胞。 在非T、非B急性 淋巴母细胞白血病细胞，几种膜抗原已被 鉴定其在淋巴细胞上的表达仅限于早期B 脉 这些基因产物的生理功能尚不清楚。 四 这些抗原中的大多数以单一多肽表示，因此似乎 由单个基因编码。 这些基因的低水平表达 产物表明它们是由RNA转录本编码的， 以低丰度存在。 因此，我们建议使用DNA介导的基因， 转移表达编码人B淋巴细胞分化的基因 小鼠细胞系中的抗原。 捐赠者的DNA序列将被 通过连续DNA转染分离并进行分子克隆。 缺乏高度重复的人DNA序列的限制性片段将被 已识别并预期包含编码序列（外显子） 在剪接的多聚腺苷酸化的信使RNA中表达。 检测这样的 cDNA文库中的序列提供了另一种克隆方法 罕见的转录本和确定它们的核苷酸序列。 第一 编码蛋白质的基因的详细表征，所述蛋白质在 克隆性造血系统恶性肿瘤具有诊断意义， 提供了必要的工具来评估这些基因的表达， 正常的B细胞发育。

The repertoire of glycoproteins expressed on the plasma membrane of lymphoid cells reflects their state of differentiation and maturation. Many of these molecules were first identified using monoclonal antibodies raised against neoplastic cells. In the case of non T, non B acute lymphoblastic leukemia cells, several membrane antigens have been identified whose expression on lymphocytes is limited to the early B lineage. The physiologic function of these gene products is unknown. Four of these antigens are represented in single polypeptides and thus appear to be encoded by single genes. The low level of expression of these gene products suggests that they are encoded by RNA transcripts which are present in low abundance. We, therefore, propose to use DNA-mediated gene transfer to express the genes encoding human B lymphocyte differentiation antigens in mouse cell lines. The donor human DNA sequences will be isolated by serial DNA transfection and will be molecularly cloned. Restriction fragments lacking highly repetitive human DNA sequences will be identified and are expected to contain the coding sequences (exons) expressed in spliced, polyadenylated messenger RNAs. The detection of such sequences in cDNA libraries provides an alternative approach to cloning rare transcripts and determining their nucleotide sequence. The first detailed characterization of genes encoding proteins whose expression in clonal hematopoietic malignancies is of diagnostic significance will provide the necessary tools to evaluate the expression of these genes in normal B cell development.