HEPATIC METABOLISM OF ALCOHOL BY GSH TRANSFERASE

拟议的工作将侧重于发展对 肝脏中的非氧化性酒精代谢，受 谷胱甘肽转移酶。具体目标包括隔离和 这些酶的纯化及其同工酶的鉴定 在肝脏中活跃地代谢酒精。研究还将包括 酒精量通过这一途径代谢的酒精量的定量 研究将通过以下方式将致癌物和酒精的共代谢联系起来 谷胱甘肽转移酶和分子遗传学研究将检查 酒精诱导谷胱甘肽转移酶的诱导机制。 这项工作代表了兰格博士的新方向，并有可能 将致癌物和酒精代谢联系起来，同时检查 改变酒精的环境影响的分子解释 肝脏内的代谢率。

The proposed work will focus on developing a molecular understanding of nonoxidative alcohol metabolism in the liver, as effected by the glutathione transferases. Specific goals include the isolation and purification of these enzymes and description of which isoenzymes are active in metabolizing alcohol in the liver. Studies will also include the quantitation of the amount of alcohol metabolized through this pathway. Studies will relate the co-metabolism of carcinogens and alcohol by the glutathione transferases and molecular genetic studies will examine the mechanism of the alcohol-induced induction of the glutathione transferases. This work represents a new direction for Dr. Lange and potentially can relate carcinogen and ethanol metabolism and, at the same time, examine molecular explanations for environmental influences on altering alcohol metabolic rates within the liver.