STAGE OF B CELL DIFFERENTIATION AND EBV GONE EXPROSSION

B 细胞分化和 EBV 表达消失的阶段

• 批准号: 3080077
• 负责人: Margaret L Gulley
• 金额: $ 8.05万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-30 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3080077
• 关键词: B lymphocyte; Burkitt's lymphoma; CD antigens; Epstein Barr virus; cell differentiation; cell growth regulation; cytogenetics; gene expression; genetic promoter element; genetic transcription; human tissue; in situ hybridization; lymphoma; nucleic acid sequence; protooncogene; tissue /cell culture; transfection; tumor promoters; viral carcinogenesis; virus genetics; virus infection mechanism

The major goals of this research project are 1) To identify EBV genes that contribute to B cell immortalization in vitro and to EBV-related lymphomagenesis in vivo, and 2) To investigate possible interactions between EBV and selected cellular genes (c-fgr protooncogene and CD23 autocrine B cell growth factor), and 3) To assess the influence of B cell differentiation on EBV-induced B cell proliferation. To accomplish our goals, malignant B cells at varying stages of differentiation will be infected with whole virus or will be transfected with selected genes (EBNA2, LMP, CD23) to identify genes that are responsible for B cell immortalization and to study the differential expression of EBV genes in early versus late stages of differentiation. These genes were chosen as most likely to immortalize based, in part, on a preliminary experiment in which we discovered high levels of transcription in an EBV-containing human lymphoma. We hypothesize that 1) Expression of one or more viral genes (possibly EBNA2 OR LMP) permit permanent growth in vitro and may contribute to viral oncogenesis in vivo; 2) EBV's effect on B cell growth could be mediated through the cellular genes c-fgr and/or CD23; and 3) B cell differentiation influences expression of EBV latent genes and outcome of EBV infection. These experiments will be carried out under the direction of Nancy Raab-Traub PhD, an established EBV researcher with expertise in molecular analysis of EBV infection. The proposed clinical investigations will be supervised by Howard Ozer MD PhD, Director of Oncology, and Dennis W. Ross MD PhD, Director of Hematopathology at the University of North Carolina at Chapel Hill.

本研究项目的主要目标是：1)鉴定EBV基因

项目成果

Immunoglobulin and T-cell receptor gene rearrangement.

免疫球蛋白和 T 细胞受体基因重排。

• 发表时间: 1994
• 期刊: Hematology/oncology clinics of North America
• 作者: Gill,JI;Gulley,ML
• 通讯作者: Gulley,ML

Diagnosis of Epstein-Barr virus associated nasopharyngeal carcinoma using fine-needle aspiration biopsy and molecular diagnostics.

使用细针抽吸活检和分子诊断诊断 Epstein-Barr 病毒相关鼻咽癌。

• DOI: 10.1002/dc.2840130216
• 发表时间: 1995
• 期刊: Diagnostic cytopathology
• 影响因子: 1.3
• 作者: Smith,SS;Fowler,LJ;Hausenfluke,L;Cho,CG;Eagan,PA;Gulley,ML
• 通讯作者: Gulley,ML

Epstein-Barr virus and lymphoproliferation in methotrexate-treated rheumatoid arthritis.

甲氨蝶呤治疗的类风湿性关节炎中的 Epstein-Barr 病毒和淋巴细胞增殖。

• 发表时间: 1996
• 期刊: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc.
• 作者: Thomason,RW;Craig,FE;Banks,PM;Sears,DL;Myerson,GE;Gulley,ML
• 通讯作者: Gulley,ML

Epstein-Barr virus-related lymphomagenesis in a child with Wiskott-Aldrich syndrome.

患有 Wiskott-Aldrich 综合征的儿童与 Epstein-Barr 病毒相关的淋巴瘤发生。

• DOI: 10.1002/hon.2900110304
• 发表时间: 1993
• 期刊: Hematological oncology
• 影响因子: 3.3
• 作者: Gulley,ML;Chen,CL;Raab-Traub,N
• 通讯作者: Raab-Traub,N

Absence of Epstein-Barr virus in lymphomatoid papulosis. An immunohistochemical and in situ hybridization study.

淋巴瘤样丘疹病中不存在 Epstein-Barr 病毒。

• DOI: 10.1001/archderm.1996.03890270051007
• 发表时间: 1996
• 期刊: Archives of dermatology
• 作者: Sangüeza,OP;Galloway,J;Eagan,PA;Braziel,RM;Gulley,ML
• 通讯作者: Gulley,ML