REGULATION OF RENAL MEMBRANE PERMEABILITY

该提议将检验膜电导的假设 是通过细胞内信使的调节来调节的， 这种调节会对两个细胞产生次要影响 膜电位与生电膜转运 流程。具体地说，此应用程序将评估 腺苷环化酶/cAMP介导的信使通路 肌醇磷脂信使途径在膜调控中的作用 灌丛边缘和膜的电导和膜传输 大鼠肾近端小管的基侧膜小泡。 此外，这项提案将决定荷尔蒙是否 这些信使通路的诱导变化导致了 隔离层的渗透率、离子电导和输运 近端小管的卷曲和直段，并在 来自这些异质肾单位的膜小泡 网站。为了实现这些目标，膜的渗透性和 电导率将用荧光和同位素进行评估。 本实验室已开发的化验方法，以及运输 将使用特定底物进行检查，这些底物 在电刷边缘或在电刷边缘中进行电生传输 基底侧膜。

The proposal will test the hypothesis that membrane conductance is regulated through the mediation of intracellular messengers, and that this regulation secondarily affects both the cell membrane potential and electrogenic membrane transport processes. Specifically, this application will assess the role of the adenylate cyclase/cAMP mediated messenger pathway and the inositol phospholipid messenger pathway in regulating membrane conductances and membrane transport in brush border and basolateral membrane vesicles of rat renal proximal tubule. Additionally, this proposal will determine whether hormonally induced changes in these messenger pathways result in changes in permeability, ionic conductances and transport in isolated convoluted and straight segments of proximal tubules, and in membrane vesicles derived from these heterogeneous nephron sites. To accomplish these goals, membrane permeabilities and conductances will be evaluated with the fluorescent and isotopic assays that have been developed in this laboratory, and transport will be examined utilizing specific substrates that are electrogenically transported in either the brush border or basolateral membranes.