CELL SURFACE IN ADHERENCE OF BACTEROIDES GINGIVALIS

虽然牙龈拟杆菌似乎在牙龈炎中起核心作用， 与牙周炎相关的复杂微生物相互作用， 基本上没有数据表明 微生物在易感宿主中建立自身， 最终参与到疾病的进程中。 细菌粘附 这是一个先决条件，既建立了许多主机- 相关微生物及其随后的毒力。 虽然B. 牙龈炎粘附于某些其它微生物， 这种类型的互动在建立 这种有机体的出现在很大程度上还未被探索。 这 研究将检测B的细菌间粘附。 牙龈炎与龈下微生物群的选定成员， 并将尝试识别和表征细胞表面 分子（即，粘附素）的潜在重要性 这种微生物的出现。 物理学家科学家奖 将为主要研究者提供教学教育 （第一阶段）和实验室培训（第一和第二阶段） 研究复杂的生物相互作用。 重要的是在 这两个阶段的培训计划，它将暴露校长 研究人员向其研究 对分子发病机理感兴趣。 研究提出， 将检查B的依从性。选择性牙龈炎 牙周微生物，已知是建立在 在B出现之前的过氧化物酶口袋。牙龈 用共聚集技术（具体目标1）。 表面 可能涉及B的结构。牙龈粘附将是 分离并进行生化和免疫学鉴定 （具体目标2）。 全面审查具体的 B的粘附。 牙龈炎对一种或多种测试菌株的敏感性将被 进行描绘这些表面分子的功能， 粘附素（特异性目标3）。 B。牙龈粘附素将是 进一步通过重组DNA技术表征， 描述或分离负责其产生的基因 （具体目标4）。 这是这项研究的长期目标（但 超出本奖项的范围），以审查的作用， 在牙周炎的体内模型中的细菌间粘附， 这可能会导致制定干预战略， 随着这种微生物在龈下的建立 环境，并可能干扰疾病的发展。

While Bacteroides gingivalis appears to play a central role in the complex microbial interactions associated with periodontitis, there are essentially no data which indicate how this microorganism establishes itself in a susceptible host to eventually participate in the disease process. Bacterial adherence is a prerequisite for both the establishment of many host- associated microorganisms and their subsequent virulence. Although B. gingivalis adheres to certain other microorganisms, the potential role of this type of interaction in the establishment and emergence of this organism remains largely unexplored. This research will examine the interbacterial adherence of B. gingivalis with selected members of the subgingival microbiota, and will attempt to identify and characterize cell surface molecules (i.e., adhesins) of potential importance in the emergence of this microorganism. The Physician-Scientist Award will provide the Principal Investigator with the didactic education (Phase I) and laboratory training (Phase I and II) required for studies on the complex biological interactions. Importantly, in both phases of this training program, it will expose the Principal Investigator to individuals and departments whose research interests are in molecular pathogenesis. The research proposed will examine the adherence of B. gingivalis to selected periodontal microorganisms which are known to be established in the peroidontal pocket prior to the emergence of B. gingivalis with a co-aggregation technique (Specific Aim 1). Surface structures potentially involved in B. gingivalis adherence will be isolated and characterized biochemically and immunologically (Specific Aim 2). A comprehensive examination of the specific adherence of B. gingivalis to one or more test strains will be carried out to delineate those surface molecules functioning as adhesins (Specific Aim 3). The B. gingivalis adhesin(s) will be further characterized by recombinant DNA technology to delineate or isolate the gene(s) responsible for their production (Specific Aim 4). It is the long range goal of this research (but beyond the scope of this award) to examine the role of interbacterial adherence in in vivo models of periodontitis, leading potentially to the development of strategies to interfere with the establishment of this microorganism in the subgingival environment, and possibly to interfere with disease development.