PHOTODYNAMIC THERAPY ON EXPERIMENTAL PAPILLOMA

喉乳头状瘤生长迅速，可能危及生命， 良性肿瘤甚至有多次复发的极大倾向 经过多种外科和内科治疗后。 已证明血卟啉衍生物(Hpd)是一种重要的生物活性物质。 强大的光敏剂，可对恶性肿瘤造成破坏 当被适当波长的光激活时，肿瘤。 最初，我计划使用一种实验性的棉尾兔乳头状瘤病毒 (CRPV)诱导皮肤乳头状瘤并观察其治疗效果 使用红光(波长630 nm)的光动力疗法(PDT)。 HPD后的总光剂量、强度和曝光时间等变量 将调查注射对乳头状瘤和皮肤的反应 产生浮肿、红斑和起泡等效果。 光动力疗法的组织学过程和分子反应 CRPV诱发的皮肤乳头状瘤将在连续时间和 间隔时间长达36个月。 为确定黏膜乳头状瘤是否保留HPD，兔口腔乳头状瘤 将使用病毒模型。定性数据将通过荧光法获得 技术，而定量数据将使用铟-11收集 标有火奴鲁鲁警局。 HPD对显性和潜伏性病毒感染的影响将是 通过分子生物学技术进行评估。

Laryngeal papillomas are rapidly growing, potentially life threatening, benign tumors which have a great propensity for multiple recurrences even after treatment by a multitude of surgical and medical regimes. It has been demonstrated that hematophorphyrin derivative (HPD) is a powerful photosensitizing agent that can cause the destruction of malignant neoplasms when activated by light of appropriate wavelength. Initially I plan to use an experimental cottontail rabbit papilloma virus (CRPV) to induce skin papillomas and to see the efficacy of treatment with photodynamic therapy (PDT) using a red light (wavelength 630 nm). Variables such as total light dose, intensity and exposure time post-HPD injection will be investigated against papilloma tumor response and dermal effects such as edema, erythema and blistering. The histologic process and molecular response which occurs with PDT treated CRPV induced skin papilloma will be investigated at serial time and intervals extending to 36 months. To establish if mucosal papilloma retain HPD, the rabbit oral papilloma virus model will be used. Qualitative data will be obtained by fluorescent techniques, whereas quantitative data will be gathered using Indium-11 labeled HPD. The effects of HPD on the visible and latent viral infection will be evaluated by molecular biologic techniques.