THY-1 RECEPTOR AND CNS NEURITE REGENERATION

如果中枢神经系统中的神经细胞能够再生 突起(轴突和树突)，就有可能治疗损伤。 例如，由于中风引起的这些过程。这份提案描述了一个 一类物质，最近被认为对 哺乳动物中枢神经系统的再生是因为在 组织培养和体内培养。THY-1是大鼠体内含量最丰富的糖蛋白 神经元；同系物已在从无脊椎动物到 天哪。已有研究表明，特异性Thy-1抗体可促进再生 哺乳动物视网膜神经节细胞的突起(也称为突起)。 THY-1抗体也促进了视神经的生长 横切面。其他类型的中枢神经元的生长似乎也 受到这些抗体的影响(几乎所有神经元都有Thy-1 其表面膜上的糖蛋白)。因此，建议 存在一种天然存在的Thy-L受体，它类似于 THY-1抗体。这种Thy-1受体可能影响发育和 中枢哺乳动物神经元对神经元突起的再生，如 视网膜神经节细胞，通过与表面Thy-1分子结合 与Thy-1抗体的作用方式相同。目前的拨款 建议用两个探针纯化内源性Thy-L受体 抗Thy-1抗体的单抗独特型抗体及其最新进展 Thy-1分子的纯化形式。Thy-L受体已经 在初步实验中用免疫荧光法初步鉴定 技术、等电聚焦和分子生物学方法。这个 人类中枢神经系统神经元上存在Thy-1的同源物表明这些 研究可能与中风后中枢神经系统再生问题有关 在人类身上。

If nerve cells in the central nervous system could regenerate their processes (axons and dendrites), it would become possible to treat injuries to these processes due, for example, to stroke. This proposal describes one class of substances that has recently been recognized to be important in regeneration in the mammalian central nervous system because of work in tissue culture and in vivo. Thy-1 is the most abundant glycoprotein in rat neurons; homologues have been identified in species from invertebrates to man. Specific Thy-1 antibodies have been shown to promote regeneration of processes (also termed neurites) by mammalian retinal ganglion cells. Thy-1 antibodies have, as well, promoted optic nerve growth after transection. The growth of other types of central neurons also appears to be influenced by these antibodies (nearly all neurons have Thy-1 glycoprotein on their surface membrane). It is therefore proposed that there exists a naturally occurring Thy-l-receptor that is analogous to the Thy-1 antibodies. This Thy-1-receptor may influence the outgrowth and regeneration of neuronal processes by central mammalian neurons, such as retinal ganglion cells, by binding to the Thy-1 molecule on the surface of neurons in the same way that the Thy-1 antibody does. The current grant proposes to purify an endogenous Thy-l-receptor by using as probes two monoclonal anti-idiotype antibodies against Thy-1 antibodies and a recently purified form of the Thy-1 molecule. The Thy-l-receptor has already been tentatively identified in preliminary experiments using immunofluorescence techniques, isoelectric focusing, and molecular biological approaches. The presence of a homologue of Thy-1 on human CNS neurons indicates that these studies may have relevance to the problem of CNS regeneration after stroke in man.