IN SITU HYBRIDIZATION STUDY OF MYELOID GENE EXPRESSION

癌基因似乎在肿瘤的发生过程中起着重要作用。 细胞的增殖和分化。技术问题 限制了对它们在正常髓系中表达的研究 细胞。原位杂交技术使分析 单个细胞的基因表达。我们建议将 正常人和白血病的原位杂交技术 髓系细胞。这将允许比较以下各项的表达 癌基因在正常髓系细胞中的表达 白血病细胞与白血病癌基因表达的相关性 它们的形态、组织化学和染色体 特点。 将建立髓系细胞分化的cDNA库 从这个文库中获得一个表达增加的基因，以及一个 髓系分化过程中表达降低的基因 将被克隆。这些基因将被表征和测序， 并将氨基酸序列和控制区进行比较 与其他已知蛋白质的同源性。这些基因将被用来 构建原位杂交探针，并对其进行表达 将研究正常髓系细胞和白血病细胞的基因。

Oncogenes appear to play important roles in the processes of cellular proliferation and differentiation. Technical problems have limited the study of their expression by normal myeloid cells. The techniques of in situ hybridization allow the analysis of gene expression by individual cells. We propose to apply the techniques of in situ hybridization to normal and leukemic myeloid cells. This will permit comparison of the expression of oncogenes by normal myeloid cells with the expression by leukemic cells, and a correlation of leukemic oncogene expression with their morphologic, histochemical, and chromosomal characteristics. A cDNA library for myeloid cell differentiation will be created and from this library a gene whose expression is increased, and a gene whose expression is decrease during myeloid differentiation will be cloned. These genes will be characterized and sequenced, and the amino acid sequence and control regions will be compared to those of other known proteins. These genes will be used to create probes for in situ hybridization, and the expression of these genes by normal myeloid and leukemic cells will be studied.