BIOGENESIS OF CANALICULAR MEMBRANE GLYCOPROTEINS

本研究提案描述了一项计划， 大鼠肝细胞膜糖蛋白胞内生物合成途径 与牛磺胆酸盐胆汁酸转运有关， 在顶端或胆小管膜极化肝细胞。 的 该分子的生物起源将使用代谢标记进行分析， 与亚细胞分级分离、免疫吸附和 免疫印迹。 通过这种方法，牛磺胆酸转运蛋白 将遵循其糖基化在高尔基体的正弦 膜，然后到胆小管膜， 糖蛋白执行其功能。 要回答的问题是 "这种在胆汁中起作用的糖蛋白 小管，从具有其他功能的糖蛋白中分离出来， 局限于窦膜吗"这个问题的答案 将阐明我们对细胞内糖蛋白生物合成的理解 并为今后的调查奠定基础。 肝胆疾病的发病机制。

This research proposal describes a plan to characterize the intracellular biosynthetic pathway of a rat liver membrane glycoprotein implicated in taurocholate bile acid transport and present in the polarized hepatocyte at the apical or bile canalicular membrane. The biogenesis of this molecule will be analyzed using metabolic labeling in combination with subcellular fractionation, immunoadsorption and immunoblotting. With such an approach, the taurocholate transporter will be followed from its glycosylatin in the Golgi to the sinusoidal membrane and then to the bile canalicular membrane where this glycoprotein carries out its unction. The question tap be answered is "At what point does this glycoprotein, which functions at the bile canaliculus, sort itself from glycoproteins with other functions that are restricted to the sinusoidal membrane?" Answers to this question will clarify our understanding of intracellular glycoprotein biogenesis and establish a foundation for future investigation into the pathogenesis of hepatobiliary disease.