VIRUS-CELL INTERACTION AND INITIATION OF INFECTION

病毒与细胞的相互作用和感染的启动

• 批准号: 3085253
• 负责人: RENE E SANTOS
• 金额: $ 5.47万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3085253
• 关键词: Alphaherpesvirinae; Herpes simplex disease; affinity chromatography; binding proteins; cell cell interaction; cell fusion; chromosome deletion; clone cells; endonuclease; gene deletion mutation; gene mutation; genetic manipulation; glycoproteins; latent virus infection; lethal genes; liposomes; molecular genetics; monoclonal antibody; nucleic acid hybridization; nucleic acid probes; plasmids; protein sequence; receptor mediated endocytosis; surface antigens; virus antigen; virus envelope; virus infection mechanism; virus morphology; virus protein; virus receptors; virus virus interaction

The objective of this training program are two-fold. First, the applicant, who has had extensive training in internal medicine and infectious diseases, wishes to devote significant time and effort to basic research training to enable him to establish an independent research program on molecular aspects of viral pathogenesis. The training program planned includes a set of courses typical of those taken by Virology and Genetics graduate students; research meetings, journal clubs and seminars; and a research program designed to provide training in the strategies and technology of virology, molecular biology, genetics and cell biology. Second, the applicant wishes to accomplish certain objectives in the research program planned. These are to define the roles of one of the essential glycoproteins of herpes simplex virus in virion morphogenesis, virion adsorption to cells, viral penetration into cells and virus-induced cell fusion (phase I). In phase II, the objectives will be to identify and characterize the cell surface components with which this glycoprotein interacts. The long term goals of this work are to increase our understanding of the pathogenesis of herpes simplex virus infections and to develop strategies for its prevention and treatment.

该培训计划的目标是双重的。 一是 申请人，谁有广泛的培训，在内科和 传染病，希望投入大量的时间和精力， 基础研究培训，使他能够建立一个 病毒分子方面独立研究计划 发病机制 计划中的培训方案包括一套 病毒学和遗传学研究生典型的课程 学生;研究会议，期刊俱乐部和研讨会;以及 旨在提供战略培训的研究计划 病毒学、分子生物学、遗传学和细胞技术 生物学 第二，申请人希望完成某些 研究计划中的目标。 这些是为了定义 单纯疱疹的一种重要糖蛋白的作用 病毒粒子形态发生中的病毒，病毒粒子对细胞的吸附，病毒 渗透入细胞和病毒诱导的细胞融合（I期）。 在 第二阶段，目标将是确定和描述 与这种糖蛋白相互作用的细胞表面成分。 这项工作的长期目标是增加我们对 单纯疱疹病毒感染的发病机制， 制定预防和治疗战略。