Translation regulation elements in both the 5` and 3` untranslated region; how do they coexist?

5`和3`非翻译区的翻译调控元件；

• 批准号: BB/F019017/2
• 负责人: Martin Bushell
• 金额: $ 13.49万
• 依托单位: MRC Toxicology Unit
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF019017%2F2
• 关键词: Translation; regulation; elements; both; untranslated

Recently, a completely new way of controlling gene expression has been identified. This has come to light after the discovery of a whole new class of genes which, unlike most genes, do not produce proteins. Instead they make very small RNA molecules, called microRNAs. There are at least 800 microRNAs within the human genome which have different effects. They work by binding to the messenger RNA of other genes and inhibiting the production of the proteins made from these genes a process known as translation. Each of these 800 small molecules is believed to interact with 100 other genes, thus adding to the complex regulation of the human genome. Already it has become clear that malfunction of miRNA regulation is associated with a growing list of human diseases, including cancer, diabetes, and viral infections. Other methods of regulating expression of genes at the level of translation also exist, and little is known about how these methods interact with miRNA regulation. This study will analyse the interaction between miRNAs and other control elements in regulating specific genes. It will also look for changes in this regulation that occur when cells are stressed, as many changes in translation occur under these conditions. This study will lead to a greater understanding of how gene expression is controlled and the complexity of the human genome.

最近，人们发现了一种控制基因表达的全新方法。这是在发现了一类全新的基因后才被发现的，与大多数基因不同的是，这些基因不会产生蛋白质。相反，它们制造非常小的RNA分子，称为microRNAs。人类基因组中至少有800种微小RNA具有不同的作用。它们通过与其他基因的信使RNA结合并抑制由这些基因合成的蛋白质的产生来发挥作用，这一过程被称为翻译。这800个小分子中的每一个都被认为与100个其他基因相互作用，从而增加了对人类基因组的复杂调控。已经很明显，miRNA调控的故障与越来越多的人类疾病有关，包括癌症、糖尿病和病毒感染。也存在在翻译水平上调节基因表达的其他方法，但对这些方法如何与miRNA调节相互作用知之甚少。本研究将分析miRNAs与其他调控元件在调节特定基因方面的相互作用。它还将寻找当细胞受到压力时这一调节的变化，因为在这些条件下翻译会发生许多变化。这项研究将使人们更好地了解基因表达是如何控制的，以及人类基因组的复杂性。

项目成果

Programming of adipose tissue miR-483-3p and GDF-3 expression by maternal diet in type 2 diabetes.

2 型糖尿病中母亲饮食对脂肪组织 miR-483-3p 和 GDF-3 表达的编程。

• DOI: 10.17863/cam.10501
• 发表时间: 2012
• 作者: Ferland-McCollough D