ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
批准号:
3115980
负责人:
ANDRAS K. SZAKAL
金额:
$8.95万
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-09-30 至 1986-08-31
关键词:
aging animal old age antibody formation antigen antibody reaction antigens body physical characteristic cell age cell components cellular immunity cytology electron microscopy humoral immunity immune complex immune complex diseases immunodeficiency juvenile animal lymph nodes lymphocyte membrane activity membrane permeability plaque assay scanning electron microscopy spleen surface antigens
中文摘要
滤泡树突状细胞 (FDC) 位于 B 细胞区室中
淋巴结和脾脏。 FDC 是非吞噬细胞,其基础
形态和表面标记代表了不同于细胞的独特细胞
淋巴细胞和巨噬细胞。 FDC 的功能还区分
它们来自其他白细胞,包括其他树突状细胞,是它们的
将免疫复合物捕获在其表面的能力。 FDC 保留这些
长时间免疫复合物。 FDC 的这些特点
构成B记忆细胞发育和长期发育的要求
长期维持免疫力。 抗原捕获的机制包括
将抗原从注射部位转运至淋巴
淋巴结滤泡由一组抗原转运细胞组成
(ATC)。 这些 ATC 是非吞噬性树突状细胞,携带
其表面的免疫复合物。 ATC 被认为代表
前 FDC 或将抗原转运至 FDC 的细胞。 数据
我们的实验室支持抗原持续存在于 FDC 上的概念
在抗体反馈系统中发挥作用,维持 B 细胞记忆并
调节体内血清抗体水平。 我们观察到老老鼠
独立研究中的实验室缺乏抗原运输
淋巴滤泡。 这些观察结果与有关报告相关
年老小鼠维持 B 记忆和抗体产生的能力下降。
为了解释这种免疫缺陷,我们提出了这样的假设:
除了 T 细胞和 B 细胞区室的缺陷(至少部分),
年老小鼠体液免疫系统的缺陷可能与
抗原运输缺陷的结果。 为了检验这个假设,
我们建议研究抗原转运缺陷的原因
这种缺陷在 B 细胞记忆维持减少中的作用
抗体的产生。 具体来说,我们计划获得定量的
观察到的缺陷的细胞学和功能数据并使用它
信息以实现修复此问题的长期目标
免疫缺陷。 为此,我们建议使用细胞转移
使用隔离的 ATC/FDC 群体的模型,我们的实验室是
与骨髓前体和/或 T 结合具有独特的资格
B 细胞或其亚群。
英文摘要
Follicular dendritic cells (FDCs) are located in the B-cell compartment of
lymph nodes and spleens. FDCs are non-phagocytic cells, which on the basis
of morphology and surface markers represent unique cells distinct from
lymphocytes and macrophages. The function of FDCs which also distinguish
them from other leukocytes, including other dendritic cells, is their
ability to trap immune complexes on their surface. FDCs retain these
immune complexes for long periods of time. These features of FDCs
constitute a requirement for the development of B memory cells and the long
term maintenance of immunity. The mechanism of antigen trapping involves
the transport of antigen from the site of its injection to lymphoid
follicles in the lymph node by a group of antigen transporting cells
(ATCs). These ATCs are non-phagocytic, dendritic-like cells which carry
the immune complexes on their surface. ATCs are thought to represent
pre-FDCs or alternatively cells transporting the antigens to FDCs. Data
from our laboratories support the concept that antigen persisting on FDCs
functions in an antibody feedback system to maintain B cell memory and to
regulate serum antibody levels in vivo. Old mice were observed in our
laboratories in independent studies to lack the transport of antigen to
lymphoid follicles. These observations correlate with reports on the
reduced capacity of old mice to maintain B memory and antibody production.
To explain this immunological deficit we proposed the hypothesis that in
addition to deficits in the T and B cell compartments, at least in part,
the deficiencies of the humoral immune system in old mice may be a
consequence of the defective antigen transport. To test this hypothesis,
we proposed to study the reasons for the defective antigen transport and
the role of this defect in the reduced maintenance of B cell memory and
antibody production. Specifically, we plan to obtain quantitative
cytological and functional data on the observed defects and use this
information to attain the long term objective of repairing this
immunological deficit. For this we proposed the use of cell transfer
models using isolated ATC/FDC populations, for which our laboratory is
uniquely qualified, in conjunction with bone marrow precursors and/or T and
B cells or their subsets.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
CELLULAR MECHANISMS OF GERMINAL CENTER REACTION IN AGING
-
批准号:2883811
-
项目类别:
-
资助金额:$24.72万
-
财政年份:1999
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
CELLULAR MECHANISMS OF GERMINAL CENTER REACTION IN AGING
-
批准号:6169447
-
项目类别:
-
资助金额:$23.16万
-
财政年份:1999
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
CELLULAR MECHANISMS OF GERMINAL CENTER REACTION IN AGING
-
批准号:6372376
-
项目类别:
-
资助金额:$23.67万
-
财政年份:1999
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
-
批准号:3115982
-
项目类别:
-
资助金额:$8.33万
-
财政年份:1985
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
-
批准号:3115987
-
项目类别:
-
资助金额:$9.06万
-
财政年份:1985
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
-
批准号:2049028
-
项目类别:
-
资助金额:$13.42万
-
财政年份:1985
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
-
批准号:3115988
-
项目类别:
-
资助金额:$12.99万
-
财政年份:1985
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
-
批准号:3115985
-
项目类别:
-
资助金额:$13.69万
-
财政年份:1985
-
负责人:ANDRAS K. SZAKAL
-
依托单位: