ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
批准号:
3115980
负责人:
ANDRAS K. SZAKAL
金额:
$8.95万
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-09-30 至 1986-08-31
关键词:
aging animal old age antibody formation antigen antibody reaction antigens body physical characteristic cell age cell components cellular immunity cytology electron microscopy humoral immunity immune complex immune complex diseases immunodeficiency juvenile animal lymph nodes lymphocyte membrane activity membrane permeability plaque assay scanning electron microscopy spleen surface antigens
中文摘要
滤泡树突状细胞(FDC)位于B细胞区室,
淋巴结和脾脏。 FDC是非吞噬细胞,其基于
形态和表面标记代表独特的细胞,
淋巴细胞和巨噬细胞。 FDCs的功能也区分
从其他白细胞,包括其他树突状细胞,是他们的
在其表面捕获免疫复合物的能力。 FDCs保留这些
免疫复合物很长时间。 FDCs的这些特点
构成了B存储单元的发展的要求,
长期保持免疫力。 抗原捕获的机制涉及
将抗原从注射部位转运至淋巴细胞
淋巴结中的滤泡通过一组抗原转运细胞
(ATC)。 这些ATC是非吞噬性的树突状细胞,其携带
它们表面的免疫复合物。 ATC被认为代表了
前FDC或可替代地将抗原转运至FDC的细胞。 数据
我们实验室的结果支持了这样一个概念,即抗原在FDC上持续存在,
在抗体反馈系统中起作用,以维持B细胞记忆,
调节体内血清抗体水平。 在我们的实验室里观察了老年小鼠,
实验室在独立的研究,缺乏运输抗原,
淋巴滤泡 这些观察结果与关于
老年小鼠维持B记忆和抗体产生的能力降低。
为了解释这种免疫缺陷,我们提出了一个假设,
除了T和B细胞区室中的缺陷外,至少部分地,
老年小鼠体液免疫系统的缺陷可能是
这是抗原转运缺陷的结果。 为了验证这个假设,
我们建议研究抗原转运缺陷的原因,
这种缺陷在降低B细胞记忆维持中的作用,
抗体生产。 具体而言,我们计划获得定量
观察到的缺陷的细胞学和功能数据,并使用此
信息,以实现修复这一长期目标,
免疫缺陷 为此,我们建议使用细胞转移
模型使用隔离的ATC/FDC人群,我们的实验室是
唯一合格的,与骨髓前体和/或T和
B细胞或其亚群。
英文摘要
Follicular dendritic cells (FDCs) are located in the B-cell compartment of
lymph nodes and spleens. FDCs are non-phagocytic cells, which on the basis
of morphology and surface markers represent unique cells distinct from
lymphocytes and macrophages. The function of FDCs which also distinguish
them from other leukocytes, including other dendritic cells, is their
ability to trap immune complexes on their surface. FDCs retain these
immune complexes for long periods of time. These features of FDCs
constitute a requirement for the development of B memory cells and the long
term maintenance of immunity. The mechanism of antigen trapping involves
the transport of antigen from the site of its injection to lymphoid
follicles in the lymph node by a group of antigen transporting cells
(ATCs). These ATCs are non-phagocytic, dendritic-like cells which carry
the immune complexes on their surface. ATCs are thought to represent
pre-FDCs or alternatively cells transporting the antigens to FDCs. Data
from our laboratories support the concept that antigen persisting on FDCs
functions in an antibody feedback system to maintain B cell memory and to
regulate serum antibody levels in vivo. Old mice were observed in our
laboratories in independent studies to lack the transport of antigen to
lymphoid follicles. These observations correlate with reports on the
reduced capacity of old mice to maintain B memory and antibody production.
To explain this immunological deficit we proposed the hypothesis that in
addition to deficits in the T and B cell compartments, at least in part,
the deficiencies of the humoral immune system in old mice may be a
consequence of the defective antigen transport. To test this hypothesis,
we proposed to study the reasons for the defective antigen transport and
the role of this defect in the reduced maintenance of B cell memory and
antibody production. Specifically, we plan to obtain quantitative
cytological and functional data on the observed defects and use this
information to attain the long term objective of repairing this
immunological deficit. For this we proposed the use of cell transfer
models using isolated ATC/FDC populations, for which our laboratory is
uniquely qualified, in conjunction with bone marrow precursors and/or T and
B cells or their subsets.
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CELLULAR MECHANISMS OF GERMINAL CENTER REACTION IN AGING
-
批准号:2883811
-
项目类别:
-
资助金额:$24.72万
-
财政年份:1999
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
CELLULAR MECHANISMS OF GERMINAL CENTER REACTION IN AGING
-
批准号:6169447
-
项目类别:
-
资助金额:$23.16万
-
财政年份:1999
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
CELLULAR MECHANISMS OF GERMINAL CENTER REACTION IN AGING
-
批准号:6372376
-
项目类别:
-
资助金额:$23.67万
-
财政年份:1999
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
-
批准号:3115982
-
项目类别:
-
资助金额:$8.33万
-
财政年份:1985
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
-
批准号:3115987
-
项目类别:
-
资助金额:$9.06万
-
财政年份:1985
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
-
批准号:2049028
-
项目类别:
-
资助金额:$13.42万
-
财政年份:1985
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
-
批准号:3115988
-
项目类别:
-
资助金额:$12.99万
-
财政年份:1985
-
负责人:ANDRAS K. SZAKAL
-
依托单位:
ROLE OF ANTIGEN TRANSPORT BY DENDRITIC CELLS IN AGING
-
批准号:3115985
-
项目类别:
-
资助金额:$13.69万
-
财政年份:1985
-
负责人:ANDRAS K. SZAKAL
-
依托单位: