IMMUNOGENETICS OF TRICHINELLA SPIRALIS IN THE MOUSE

已知有两个H-2基因ts-1和ts-2影响免疫反应 在小鼠体内对抗旋毛虫。有证据表明T-T细胞 相互作用是产生有效免疫反应所必需的，而且 易感小鼠在相互作用的其中一个中表达ts-2相关缺陷 T细胞群。基因特性化的免疫遗传学实验 H-2同源品系小鼠将确定T-T细胞是否 有效的反应需要相互作用，描述细胞的特征 并鉴定表达ts-2相关基因的细胞群。 叛逃。为了实现这些目标，选定的淋巴结细胞群体 会被注射到具有基因特征的、T细胞缺陷或正常的 老鼠。然后这些小鼠将感染旋毛虫和它们的水平 所测得的阻力。T细胞缺陷的细胞接受者将是 胸腺切除，照射，并用同基因骨髓重建。 用于转移的受过旋毛虫教育的T细胞将来自于辐射， 胸腺细胞重组小鼠或来自感染T. 螺旋藻。培养旋毛虫的细胞将被注射到接受者体内 单独或与从Normal中选择的细胞群组合 老鼠。表达的抗Thy-1和Ly抗原的单抗 T细胞和B细胞、巨噬细胞和 T细胞亚群将被用来仔细定义组成 被转移的细胞数量。这些活体实验的结果将是 通过研究细胞与细胞之间的相互作用来证实 对高纯度保护剂的最大体外增殖反应 诱导旋毛虫抗原。以补充上述研究，并 进一步探讨T细胞和抗体在免疫中的作用 对旋毛虫的反应，我们将测试注射的体内效果 用克隆的T细胞和特异性的单抗感染小鼠 旋毛虫保护性诱导抗原。寄生虫诱导的作用 同样，免疫抑制也将通过给小鼠注射 旋毛虫分泌特异性I-J+抑制因子的制备 抑制性T细胞杂交瘤。单抗、T细胞克隆和 抑制因子也将被用来纯化和鉴定相关的T. 螺旋虫抗原。

Two H-2 genes, Ts-1 and Ts-2, are known to influence the immune response against T. spiralis in the mouse. Evidence suggests that T-T cell interaction is required to generate an efficient immune response, and that susceptible mice express a Ts-2 associated defect in one of the interacting T cell populations. Immunogenetic experiments in genetically characterized H-2 congenic strains of mice will establish whether or not T-T cell interaction is required for an efficient response, characterize the cells involved, and identify the cell population expressing the Ts-2-associated defect. To accomplish these aims, selected populations of lymph node cells will be injected into genetically characterized, T cell deficient or normal mice. These mice will then be infected with T. spiralis and their levels of resistance measured. T cell deficient recipients of cells will be thymectomized, irradiated, and reconstituted with syngeneic bone marrow. T. spiralis-educated T cells used for transfer will be from irradiated, thymocyte reconstituted mice or from normal mice infected with T. spiralis. T. spiralis-educated cells will be injected into recipients either alone or in combination with selected cell populations from normal mice. Monoclonal antibody specific for the Thy-1 and Ly antigens expressed on T cells and for the Ia antigens expressed on B cells, macrophages and subpopulations of T cells will be used to carefully define the composition of cells being transferred. Results of these in vivo experiments will be confirmed by studying the cell-cell interactions required to generate a maximal in vitro proliferation response to highly purified, protection inducing T. spiralis antigens. To complement the above studies and to further explore the role of T cells and antibody in effecting the immune response against T. spiralis, we will test the in vivo effect of injecting mice with cloned T cells and with monoclonal antibody specific for protection inducing antigens of T. spiralis. The role of parasite-induced immunosuppression will likewise be explored by injecting mice with preparations of T. spiralis-specific I-J+ suppressor factors secreted by suppressor T cell hybridomas. Monoclonal antibody, T cell clones and suppressor factors will also be used to purify and characterize relevant T. spiralis antigens.