PHYSICAL CHEMISTRY OF VIRUSES AND THEIR COMPONENTS

病毒及其成分的物理化学

• 批准号: 3124485
• 负责人: LOREN A DAY
• 金额: $ 22万
• 依托单位: PUBLIC HEALTH RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3124485
• 关键词: analytical ultracentrifugation; bacterial virus; circular dichroism; densitometry; electron microscopy; fluorescence spectrometry; genome; light scattering; nucleic acid structure; virus DNA; virus genetics; virus morphology; virus replication

We propose structural studies on most available filamentous bacterial viruses, on their intracellular precursor complexes between DNA binding proteins and progeny DNAs, and on the genomes of some. All of the viruses have Gram-negative bacteria as hosts, several of which are pathogenic either in plants or animals. All of the viruses have the same basic overall morphology and all contain a single-stranded DNA genome packed in a sheath of thousands of identical subunits. Techniques such as ultracentrifugation, electron microscopy, light scattering, CD and fluorescence spectroscopy, X-ray diffraction, and other techniques will be applied to determine fundamental structure parameters. Studies on interactions, between intact virions between various virion components, and between the DNA binding proteins and nucleic acids are also proposed. The amino acid sequences of the major structural proteins are to be established through DNA sequencing and protein sequencing techniques. Theoretical work will be directed toward unified theories of the structures of these similar, but in detail diverse, nucleoproteins having the same function, and toward understanding of their evolutionary relationships.

我们建议对大多数可用的丝状细菌进行结构研究。 病毒，对其胞内前体复合体之间的DNA结合 蛋白质和后代DNA，以及一些人的基因组上。所有的病毒 有革兰氏阴性菌作为宿主，其中几种是致病的 无论是植物还是动物。所有的病毒都有相同的基本特征 整体形态和都包含一个单链DNA基因组包装在一个 由数千个相同的亚基组成的鞘。技术，如 超速离心、电子显微镜、光散射、CD和 荧光光谱、X射线衍射和其他技术将被 应用于确定基本结构参数。研究：关于 不同病毒粒子组件之间的完整病毒粒子之间的相互作用，以及 还提出了DNA结合蛋白和核酸之间的关系。这个 主要结构蛋白的氨基酸序列有待确定 通过DNA测序和蛋白质测序技术。理论工作 将指向这些结构的统一理论 类似的，但细节不同的，具有相同功能的核蛋白， 以及对它们进化关系的理解。