STUDIES OF TRYPANOSOMAL EXOANTIGENS

外源性抗原由致病性唾液腺和非致病性粪肠腺产生 锥虫如布氏锥虫和锥虫。刘易斯，分别。 这些 抗原引起保护性的杀锥虫反应，但其中一种外抗原 中医lewisi是一种能增强戒断反应的脱泻药， 生殖抑制免疫力，这是独特的，并负责 粪便感染的良性性质。 建议继续研究外抗原和其他相关的 这些锥虫的细胞表面的成分。 这些将包括 亲和层析法分离脱落素原 抗体IgG，并在抗原的表征后，寻找 在T.布氏杆菌;继续研究抑制素的亲合力， 在感染期间与外来抗原形成免疫复合物;分离和 对T. 继续研究所有已识别表面的盖层形成 T. 刘易斯;表面涂层形成及其可能性的研究 与T.间接免疫荧光法检测布氏杆菌， 加帽技术;和抗原性变体的细胞表面的分析， T.布氏杆菌用于共享的、可获得的抗原，其通常不是免疫原性的。 通过这种分析方法，也许可以了解为什么 针对致病性非洲锥虫的反应未能发展。 此外，拟议的研究将提供有关生物学的基本信息， 这些寄生虫，并可能揭示重要的成分，可以提供 为有效防治病原性锥虫提供依据。

Exoantigens are produced by pathogenic salivarian and nonpathogenic stercorarian trypanosomes such as Trypanosoma brucei and T. lewisi, respectively. These antigens elicit a protective, trypanocidal response, but one of the exoantigens of T. lewisi is ablastinogen, which elicits the ablastic response, a reproduction-inhibiting immunity that is unique to and responsible for the benign nature of stercorarian infections. It is proposed to continue studies of exoantigens and other associated components on the cell surface of these trypanosomes. These will include isolation of ablastinogen by affinity chromatography with recently collected ablastic IgG, and after characterization of the antigen, a search for a counterpart in T. brucei; continuation of studies of the avidity of ablastin and of immune complex formation with exoantigens during infection; isolation and characterization of the two variant specific antigens (exoantigens) of T. lewisi; continuation of studies of cap formation with all identified surface ligands of T. lewisi; a study of surface coat formation and its possible relation to antigenic variation in T. brucei by indirect immunofluorescence and capping techniques; and an analysis of the cell surface of antigenic variants of T. brucei for shared, accessible antigens that are not ordinarily immunogenic. Through this analytical approach, it may be possible to learn why an ablastic response fails to develop against the pathogenic African trypanosomes. Moreover, the proposed study will provide basic information about the biology of these parasites and may reveal significant components that could provide the basis for effective control measures against the pathogenic trypanosomes.