ORGANIZATION AND EXPRESSION OF DNA TUMOR VIRUS GENOMES

我们的目标是研究DNA肿瘤病毒的基因表达调控 分子水平上的基因组，并利用这一知识来理解 真核基因表达和病毒-宿主细胞相互作用的机制。 目的是研究腺病毒的转录调控。 5型早期转录区域(EII)，并试图分离和 表征由病毒前期EIA基因激活的宿主基因。 EIIA晚期启动子的转录控制元件将是 使用连接子扫描突变在病毒水平上进行鉴定 接近。转录调控元件的功能关系 两个DIIA(早期和晚期)启动子及其基因基础 将使用新的方法研究病毒裂解周期的差异调节 变异病毒。生物化学方法，如DNA-蛋白质的凝胶分析 涉及核酸外切酶III的复合体和体内检测将被用于 确定可能与转录因子相互作用的转录因子 两个EIIA发起人的控制要素。 利用分子杂交和基因克隆技术，尝试 将被用来识别和分离病毒激活的宿主基因 早期EIA前基因。

Our goal is to study the regulation of gene expression of DNA tumor virus genomes at the molecular level and to use this knowledge to understand the mechanisms of eukaryotic gene expression and virus-host cell interactions. The objectives are to study the transcriptional regulation of adenovirus type 5 early transcriptional region (EII) and to attempt to isolate and characterize the host genes activated by the viral pre-early EIA gene. Transcriptional control elements of the EIIA-late promoter will be identified at the virus level using the linker scanning mutagenesis approach. Functional relationship of the transcriptional control elements of the two DIIA (early and late) promoters and the genetic basis of their differential regulation in viral lytic cycle will be studied using novel mutant viruses. Biochemical approaches such as gel assays for DNA-protein complexes and in vivo assays involving exonuclease III will be employed to identify transcription factors which may interact wih the transcriptional control elements of the two EIIA promoters. Utilizing molecular hybridization and gene cloning techniques, attempts will be made to identify and isolate the host genes activated by the viral pre-early EIA gene.