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HUMAN TL (HTL) REGION IN THE HLA LINKAGE GROUP

HLA 连锁群中的人类 TL (HTL) 区域

基本信息

批准号：
3126984
负责人：
EDMOND J YUNIS
金额：
$ 8.42万
依托单位：
DANA-FARBER CANCER INST
依托单位国家：
美国
项目类别：
财政年份：
1980
资助国家：
美国
起止时间：
1980-09-30 至 1987-11-30
项目状态：
已结题

项目摘要

The objectives of this proposal are to detect and characterize the human T (HT1a) region gene products recognized by alloantisera procured from multiparous women. During the last two years, significant number of alloantisera have been characterized. Generally, two groups of differentiation alloantigens, both of which appear to be polymorphic gene products, were recognized: One group is expressed only on thymocytes, T leukemic cells and lymphoblastoid cell lines which is tentatively named HTL (TL-like), while the other group is expressed on activated lymphocytes, resting spleen cells and lymph node cells named HT (Qa-like) antigens. Both groups of alloantigens are associated with Beta2-microglobulin and are probably linked to the HLA region. Experiments will aim to better define these polymorphic alloantigens by means of serological reactivity with alloantisera and by biochemical methods utilizing monoclonal antibodies. Cross-absorption will be used in order to study the serological differences between HTL and HT gene products. High resolution isoelectrofocusing (IEF) gel electrophoresis and Western blot will be used to detmonstrate biochemical structure and genetic polymorphism of the putative alloantigens. These antigens will be obtained from radiolabelled cells by sequential immunoprecipitation using alloantisera and monoclonal antibodies to recognize different Beta2m-associated molecules. Our specific aims will be: a) to determine if HT and HTL gene products contain Beta 2m and to study serological differences between HTL and HT antigens. b) To study linkage disequlibrium between HLA and clusters of HTL gene products and to study segregation analysis of HT determinants in HLA genotyped familes. c) To demonstrate structural polymorphism of the HT and HTL gene products. d) to produce monoclonal antibodies directed against HT and HTL determinants. Our long term objectives is to define the fine structure of gene and gene products of a new genetic system which may be equivalent to the murine T1a genetic system. Such findings will be useful to extend our knowledge of the role of MHC (HLA and non-HLA) gene products in the immune regulation and in providing new reagents for tissue typing for allotransplantation.

本提案的目标是检测和表征人类T

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Characterization of human umbilical cord blood lymphocyte subsets fractionated on immobilized peanut agglutinin.

固定化花生凝集素分离的人脐带血淋巴细胞亚群的表征。

DOI：
10.1016/s0198-8859(83)80007-x
发表时间：
1983
期刊：
Human immunology
影响因子：
2.7
作者：
Rosenberg,M;Gazit,E;Sharon,N
通讯作者：
Sharon,N

Ia-like antigens in the small intestinal mucosa of normal and celiac children.

正常儿童和乳糜泻儿童小肠粘膜中的 Ia 样抗原。

DOI：
发表时间：
1985
期刊：
Israel journal of medical sciences
影响因子：
0
作者：
Avigad,S;Jonas,A;Shiner,M;Gazit,E
通讯作者：
Gazit,E

Alloantisera detect HLA-A-B-C and non-HLA differentiation antigens on human thymocytes.

同种抗血清可检测人胸腺细胞上的 HLA-A-B-C 和非 HLA 分化抗原。

DOI：
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
0
作者：
Mahoney,RJ;Gazit,E;MacCarthy-Shanley,K;Martin-Alosco,S;Palomaki,GE;Smith,DE;Yunis,EJ
通讯作者：
Yunis,EJ

Unique proliferation-associated marker expressed on activated and transformed human cells defined by monoclonal antibody.

在由单克隆抗体定义的激活和转化的人类细胞上表达的独特增殖相关标记。