IMMUNOCHEMISTRY OF TYPE II GROUP B STREPTOCOCCI

II型B族链球菌是导致严重人类感染的常见原因。 感染. 它们是母体和成人B组的主要病因 链球菌感染，仅次于III型菌株 新生儿感染 针对型特异性 B族链球菌的多糖被认为在 对这些生物体的防御作用。 然而， 保护性抗体所针对的免疫决定簇， 方向不被理解。 因此，完整的化学结构 使用甲基化分析测定II型多糖， 特定化学降解和13 C-N.M.R. 的确切性质 重要的免疫决定簇将使用各种 寡糖片段在采用单克隆抗体的ELISA中作为抑制剂 II型多糖的抗体。 此外， 天然存在的II型B族链球菌抗体将 在来自已知定植患者的选定人血清中检查 状态或感染。 将采用两种主要的抗体测定法： 用于测量抗体水平的定量ELISA，以及调理吞噬细胞的 用于评估这些抗体的功能作用的测定。

Type II group B Streptococci are a frequent cause of serious human infections. They are the major cause of maternal and adult group B streptococcal infections and are second only to type III strains as a cause of neonatal infections. Antibodies directed against the type-specific polysaccharides of group B streptococci are thought to play an important role in host defense against these organisms. However, the precise nature of the immunological determinants against which protective antibodies are directed is not understood. Therefore, the complete chemical structure of the type II polysaccharide will be determined using methylation analysis, specific chemical degradations and 13C-N.M.R. The exact nature of the importance immunological determinants will be defined using various oligosaccharide fragments as inhibitors in an ELISA employing monoclonal antibodies to the type II polysaccharide. In addition, the specificity of naturally occurring antibodies to type II group B streptococci will be examined in selected human sera from patients with known colonization status or infection. Two main antibody assays will be employed: a quantitative ELISA for measuring antibody levels, and an opsonophagocytic assay for assessing the functional role of these antibodies.