BIOCHEMICAL STUDIES OF HUMAN T CELL SURFACE MARKERS

人细胞表面抗原的分离及化学特性 胸腺来源的淋巴细胞将加强我们对分化的理解， T细胞谱系的途径。 此外，从这些信息中获得的信息 研究将提供更精确的分子事件的理解 参与衍生自人胸腺细胞的恶性细胞的发育。 拟定研究的目的是利用抗T3单克隆试剂， 抗-T4和抗-T5，它们与功能上不同的 T细胞亚群，用于分离和表征细胞表面抗原。 与T辅助/诱导亚群相关的分化抗原 (T3+，T4+，T5-）可能是介导 展示它们的细胞的特定功能。 同样，我们认为， T细胞亚群上的分化标志物被编程为 抑制/细胞毒性功能（T3+，T4-，T5+）可能在以下方面发挥作用： 免疫反应的效应阶段。 很清楚，很纯净， 特征性细胞表面抗原T3、T4和T5，此外， 这些抗原的特异性单克隆抗体， 这些功能的分子解剖。 结构非均匀性研究 这些抗原在人群中的分布将决定 遗传多态性 最后，T细胞亚群的化学描述 标记物对于未来的分子生物学研究将是至关重要的。 T细胞分化的遗传学 我们相信，对T细胞表面更详细的了解将有助于 从而更深入地了解这些在免疫系统中的功能， 反应

The isolation and chemical characterization of cell surface antigens of human thymus derived lymphocytes will enhance our understanding of the differentiative pathway of T cell lineage. In addition, the information obtained from such studies will provide a more precise understanding of the molecular events involved in the development of malignant cells derived from human thymocytes. The intention of the proposed studies is to utilize monoclonal reagents anti-T3, anti-T4 and anti-T5, which are specifically reactive with functionally distinct T cell subsets, for isolation and characterization of cell surface antigens. Differentiation antigens which are associated with the T helper/inducer subset (T3 plus, T4 plus, T5 minus) are likely to be molecules that mediate the specific functions of those cells which display them. Similarly we believe that the differentiation markers on the T cell subset programmed for the suppressor/cytotoxic functions (T3 plus, T4 minus, T5 plus) may play a role in the effector phase of the immune response. Clearly, purified, well characterized cell surface antigens T3, T4 and T5, in addition to new sets of monoclonal antibodies specific for these antigens, would be required for the molecular dissection of such functions. A study of the structural heterogeneity of these antigens in the human population would determine the extent of a genetic polymorphism. Lastly, a chemical description of the T cell subset markers would be of paramount importance for future studies of the molecular genetics of T cell differentiation. It is our belief that a more detailed knowledge of the T cell surface will result in a deeper understanding of the function of these in the immune response.