STRUCTURE AND FUNCTION OF POXVIRUS GENES

这些研究的长期目标是确定 影响痘病毒基因表达的调节。这项工作将 包括转录控制方面的研究、翻译 控制、信使核糖核酸的稳定性以及病毒基因产物对病毒的影响 宿主细胞和宿主动物。具体地说，以下将是 研究：1.晚期mRNA5‘-聚(A)先导的产生， 以及这些前导序列对翻译效率和 信使核糖核酸稳定性。2.后期定义的3‘-末端的产生 某些强表达基因的mRNAs；这些3‘端的作用 在下游转录单位上形成。3.监管 编码病毒第二大亚基的基因的表达 依赖DNA的RNA聚合酶。该附属单位的职能也将 接受检查。4.38K基因在中间时间的表达 在病毒复制周期中。其基因产物在植物生长发育中的作用 蛋白水解酶的抑制及其对病毒-宿主相互作用的影响 也将接受调查。 标准的生化和遗传程序，包括现场定向 诱变程序和一种新的遗传互补系统Will 用来实现这些目标。我们将利用独一无二的 痘病毒的性质。 这些研究将有助于我们理解分子事件 一般参与真核生物基因表达的调控， 尤其是在痘病毒中。病毒DNA依赖核糖核酸的研究 聚合酶，它在结构上与真核RNA相似 聚合酶。对38K基因产物的研究将促进我们的 了解病毒与宿主的相互作用会导致出血或 感染部位的白细胞聚集。此外，这些 研究将帮助我们开发更有效的痘病毒表达 媒介，并有助于安全有效的发展 痘病毒衍生疫苗。

The long-term objectives of these studies are to determine the mechanisms effecting the regulation of poxvirus gene expression. This work will include studies on aspects of transcriptional control, translational control, mRNA stability, and the effects of viral gene products on the host cell and the host animal. Specifically, the following will be investigated: 1. The generation of the 5'-poly(A) leaders of late mRNAs, and the effect of these leader sequences on translational efficiency and mRNA stability. 2. The generation of the defined 3'-ends of the late mRNAs of certain strongly-expressed genes; the effect of these 3'-end formation on downstream transcription units. 3. The regulation of expression of the gene encoding the second-largest sub-unit of the viral DNA- dependent RNA polymerase. The functions of this sub-unit will also be examined. 4. The expression of the 38K gene at intermediate times during the viral replication cycle. The role of its gene product in the inhibition of proteinases, and its effect on the virus-host interaction will also be investigated. Standard biochemical and genetic procedures, including site-directed mutagenesis procedures and a novel genetic complementation system, will be used to achieve these goals. Advantage will be taken of the unique properties of the poxviruses. These studies will contribute to our understanding of molecular events involved in the regulation of gene expression in eukaryotes in general, and in poxviruses in particular. Studies on the viral DNA-dependent RNA polymerase, which is structurally similar to the eukaryotic RNA polymerases. Studies on the 38K gene's product should advance our understanding of virus-host interactions resulting in hemorrhage or leukocyte accumulation at the site of the infection. In addition, these studies will help us to develop more efficient poxvirus expression vectors, and contribute to the development of safe and effective poxvirus-derived vaccines.