MOBILE DNA IN CANDIDA ALBICANS BIOLOGY AND EPIDEMIOLOGY

白色念珠菌生物学和流行病学中的移动 DNA

• 批准号: 3136317
• 负责人: STEWART SCHERER
• 金额: $ 10.45万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3136317
• 关键词: Candida albicans; DNA; biological polymorphism; candidiasis; chromosomes; communicable disease transmission; communicable diseases; fungal genetics; gel electrophoresis; gene redundancy; genetic manipulation; genetic mapping; genetic markers; genetic strain; human tissue; linkage mapping; microorganism classification; molecular cloning; nucleic acid sequence; population genetics; protoplast /spheroplast; transposon /insertion element

Candida albicans, a dimorphic yeast, is among the most commonly encountered human pathogens. Infections caused by Candida range from vaginitis to severe systemic infections in immune compromised patients. The goals of this proposal are: 1) to identify mobile DNA elements in the Candida genome; 2) to use these DNA probes as epidemiologic tools; and 3) to then apply them to basic questions about the biology of the organism. The search for mobile DNA sequences will not be confined to transposable elements but will include any DNA segments associated with elevated frequencies of DNA polymorphisms. The shared properties of movable elements from a variety of organisms suggest methods to isolate them in other species. Using as criteria dispersion, repetition, and absence from related species, a successful screen for such sequences has been conducted. Preliminary results indicate that DNA polymorphisms will be a powerful tool in the typing of C. albicans strains. Candida chromosomes are small enough to be resolved by pulsed field electrophoresis and their lengths are polymorphic as well. It should be possible to combine these facts with single copy DNA polymorphisms and genetic experiments using segregation from fused protoplasts to build a combined physical and genetic map of the organism.

白色念珠菌是一种二型酵母菌， 人类病原体 念珠菌引起的感染范围从阴道炎到 免疫功能低下患者发生严重全身感染。 的目标 本研究的主要目的是：1）鉴定念珠菌中的移动的DNA元件 基因组; 2）使用这些DNA探针作为流行病学工具; 3）然后 将它们应用于生物体的基本生物学问题。 的 对移动的DNA序列的搜索将不限于转座的 元件，但将包括与升高的 DNA多态性频率 动产共有财产 从各种生物体中提取的元素表明有方法将它们分离出来， 其他物种。 以分散、重复和缺席为标准， 相关物种，成功筛选这样的序列， 进行。 初步结果表明，DNA多态性将是一个 在C中键入的强大工具。白色念珠菌菌株。 念珠菌染色体 小到足以通过脉冲场电泳解析， 长度也是多态的。 应该可以将这些联合收割机 单拷贝DNA多态性和遗传实验的事实， 从融合的原生质体分离，以建立一个结合的物理和遗传 有机体的地图。