TRYPANOSOME VARIANT SURFACE GLYCOPROTEIN CARBOHYDRATES
TRYPANOSOME VARIANT SURFACE GLYCOPROTEIN CARBOHYDRATES
批准号:
3144680
负责人:
HERMAN VAN HALBEEK
金额:
$12.68万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-09-30 至 1994-08-31
关键词:
Trypanosoma Trypanosoma brucei rhodesiense acetylcholinesterase carbohydrate structure erythrocytes glycoprotein structure glycosylation host organism interaction human tissue laboratory mouse laboratory rat mass spectrometry microorganism culture nuclear magnetic resonance spectroscopy oligosaccharides protozoal antigen surface antigens trypanosomiasis
中文摘要
非洲锥虫病是一种影响非洲大部分地区的寄生虫病
英文摘要
African trypanosomiasis is a parasitic disease affecting large areas of the
African continent. It presents a threat to man and drastically impedes
effective cattle-rearing. The causative agents of this infectious disease
are members of the genus Trypanosoma. The parasite evades the mammalian
host's immunological response to its presence in body fluids by antigenic
variation: the expression of a sequence of immunologically distinct
glycoprotein coats over the entire surface of the cells of the invading
organism. These variant surface glycoproteins (VSGs) have one or more
N-linked glycans and are anchored in the trypanosome membrane by a
glycosyl-phosphatidyl-inositol(GPI) moiety. Of the three classes of VSGs
(distinguished on the basis of the C-terminal amino acid), the covalent
structures of the GPI anchors and N-glycans of only two VSGs, both of class
I, of only one trypanosome species (T. b. brucei) have been fully
elucidated.
The primary aim of the proposed research is to structurally characterize
the GPI anchors and N-glycans of a variety of trypanosome VSGs to determine
whether such oligosaccharides are significantly different to those of
mammalian glycoproteins. Preliminary data indicate that the GPI anchors
and the N-glycans of trypanosomes are different from those of the mammalian
host. The GPI anchors in particular are considered prime potential targets
for chemotherapeutic treatment of trypanosomiasis. We propose to
characterize the carbohydrate structures of the VSGs of six trypanosome
variant antigen types, and compare them with those of mammalian
glycoproteins. In particular we propose to:
1. Isolate the VSGs and their carbohydrate moieties from T. b.
rhodesiense LouTat 1 and 1A (both class I), and from T. b. rhodesiense
LouTat 1.5 and T. congolense YNat 1.1 and 1.3 (all class II), grown in
rodents and/or in vitro. Obtain a class III VSG and the GPI anchor from a
mammalian glycoprotein (human erythrocyte acetylcholinesterase, AChE) from
collaborators.
2. Characterize the structures of the VSG GPI anchors and compare them
with each other. Look for host influence on VSG GPI glycosylation.
Compare the structures to the structure of the GPI anchor of AChE.
3. Structurally characterize the N-glycans from the VSGs and
investigate the presence of unusual structural elements. Look for host
influence on VSG N-glycosylation.
The long-term aim is to find a target for the development of
chemotherapeutic agents. The proposed studies should provide the
foundation necessary for further research leading to the development of new
reagents for the treatment of African trypanosomiasis.
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科研奖励(0)
会议论文
SURFACE CARBOHYDRATES OF PNEUMOCYSTIS CARINII
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批准号:2067789
-
项目类别:
-
资助金额:$16.77万
-
财政年份:1992
-
负责人:HERMAN VAN HALBEEK
-
依托单位:
TRYPANOSOME VARIANT SURFACE GLYCOPROTEIN CARBOHYDRATES
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批准号:2065209
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项目类别:
-
资助金额:$12.39万
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财政年份:1991
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负责人:HERMAN VAN HALBEEK
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依托单位:
CRYPTOCOCCUS NEOFORMAN POLYSACCHARIDE ANTIGENIC EPITOPE CONFORMATION: AIDS
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批准号:5225060
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:HERMAN VAN HALBEEK
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依托单位:--
LIPOPHOSPHOGLYCANS (LPG) FROM LEISHMANIA DONOVANI & LEISHMANIA MEXICANA
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批准号:5225062
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:HERMAN VAN HALBEEK
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依托单位:--
CARBOHYDRATE STRUCTURES OF COBRA VENOM FACTOR GLYCOPROTEIN
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批准号:5225061
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:HERMAN VAN HALBEEK
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依托单位:--
CHO CELLS EXPRESSED RECOMBINANT GP120 N GLYCOSYLATED SITE MAPPING: HIV
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批准号:5225035
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:HERMAN VAN HALBEEK
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依托单位:--
MULTIDIMENSIONAL NMR SPECTROSCOPY AT GEORGIA STATE UNIVERSITY
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批准号:5225104
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:HERMAN VAN HALBEEK
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依托单位:--
N ACETYLLACTOSAMINE OLIGOMERS ENZYMATIC SYNTHESIS: L 14 INTERACTION STUDY
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批准号:5225034
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:HERMAN VAN HALBEEK
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依托单位:--
INTERACTIONS OF GALECTIN 1 W/ LACTOSAMINE TYPE OLIGOSACCHARIDES
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批准号:5225032
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:HERMAN VAN HALBEEK
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依托单位:--
CONFORMATION ANALYSIS OF SEROTRANSFERRIN DERIVED GLYCOPEPTIDES
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批准号:5225033
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:HERMAN VAN HALBEEK
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依托单位:--
HUMAN MONOCLONAL SPECIFIC ANTI LPS IGM AB & POLYSPECIFIC IGM CARBOHYDRATE STRUCT
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批准号:5225063
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项目类别:
-
资助金额:$0.0万
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财政年份:--
-
负责人:HERMAN VAN HALBEEK
-
依托单位:--