BIOCHEMISTRY OF THE ADRENAL MEDULLA

肾上腺髓质的生物化学

• 批准号: 3150715
• 负责人: NORMAN KIRSHNER
• 金额: $ 14.41万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1975
• 资助国家: 美国
• 起止时间: 1975-05-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3150715
• 关键词: adenosinetriphosphatase; adrenal medulla; catecholamine inhibitor; catecholamines; digitonin; magnesium; membrane permeability; neural information processing; neurochemistry; neurotransmitter biosynthesis; nicotine; radiotracer; secretion; sympathetic nervous system; tissue /cell culture

Primary cultures of adrenal medulla cells will be used to investigate cellular and molecular events concerned with stimulus-secretion coupling and other aspects of adrenal medulla function. Radioligand binding studies will be used to characterize the muscarinic and nicotinic receptors on the plasma membrane and their interaction with various agonist and antagonists determined. These studies will provide a better understanding of the site of action of various secretory antagonists and the interaction of muscarinic and nicotinic agonists on the same cell. A simplified model system using cells permeabilized by treatment with digitonin will be developed. This system may enable the identification and characterization of intracellular components involved in stimulus-secretion coupling. The role of Na+:Ca++ countertransport and energy-dependent Ca++-pumping in terminating agonist-stimulated catecholamine secretion will be elucideated. The presence of a Ca++-activated Mg++-ATPase in the plasma membrane will be investigated and its molecular properties and physiological defined. The primary cultures also provide a good model system to better characterize the uptake 1 system for transport of catecholamines across the plasma membrane and to study the role of secretory agonists in regulating its activity. The regulation of catecholamine biosynthesis will also be investigated. These studies are not only relevant to the adrenal medulla but also to the physiology and function of the sympathetic nervous system.

原代培养的肾上腺髓质细胞将用于研究 与刺激-分泌偶联有关的细胞和分子事件 以及肾上腺髓质功能的其他方面。 放射性配体结合研究 将被用来表征毒蕈碱和烟碱受体上的 质膜及其与各种激动剂和拮抗剂相互作用 测定 这些研究将使人们更好地了解该网站 各种分泌拮抗剂的作用和相互作用 毒蕈碱和烟碱激动剂在同一细胞上。 简化模型 使用通过毛地黄皂苷处理而透化的细胞的系统将 开发 该系统可以使识别和表征 参与刺激分泌偶联的细胞内成分。 的 Na+：Ca++逆向转运和能量依赖性Ca++泵在 终止激动剂刺激的儿茶酚胺分泌将是 阐明。 血浆中Ca ~（++）激活的Mg ~（++）-ATPase的存在 膜将被研究，其分子特性和 生理定义。 原代培养物也提供了一个很好的模型 系统，以更好地表征用于运输的摄取1系统 的作用，并研究其作用。 分泌激动剂调节其活性。 调控 还将研究儿茶酚胺的生物合成。 这些研究 不仅与肾上腺髓质有关， 交感神经系统的功能。