MECHANISM AND REGULATION OF CONTRACTILE PROTEINS
MECHANISM AND REGULATION OF CONTRACTILE PROTEINS
批准号:
3151574
负责人:
DAVID Daniel HACKNEY
金额:
$9.94万
依托单位国家:
美国
项目类别:
财政年份:
1979
资助国家:
美国
项目状态:
已结题
起止时间:
1979-08-01 至 1990-07-31
中文摘要
这项研究的长期目标是更好地理解
收缩过程的潜在机制及其调节。
收缩是许多生物系统的一个重要性质。
除了骨骼肌之外。皮肤上的光滑肌肉
胃肠道提供必要的刺激,以适当
消化和循环系统中的平滑肌肉起着主要作用。
角色。非肌肉细胞中的收缩蛋白被认为参与了
在细胞分裂、胞质流动、吞噬等过程中
需要修改和维护细胞形状的。收缩,收缩
血小板细胞在血栓成熟过程中起着重要作用。
这个项目沿着几条主要路线进行。第一个涉及到
对骨骼肌系统有更好的了解。这
肌肉是人们最了解的收缩系统,但仍有许多问题有待解决。
确定的因素包括丝状组织和
肌动蛋白和肌球蛋白相互作用的详细性质和控制。一个
第二个主要研究方向涉及到其他收缩技术的应用。
与骨骼肌一起发展的技术系统。顺畅的
将对鸡胆的肌肉进行初步研究。其他可能的情况
应用包括血小板的非肌肉收缩系统。这个
第三个主要研究方向是使用收缩蛋白作为
发展对影响的总体理解的模型系统
酶催化及其超分子酶部位的调控
结构。这是一个问题,它是扩展
从研究酶学在体外稀溶液中的反应转变为
在体内发生的反应的复杂性。
将广泛使用同位素标记的交换反应
水和三磷酸腺苷水解产生的磷酸盐之间的氧原子。
对这种交换反应的分析为我们提供了对
骨骼肌球蛋白的机制,并将继续这样做。它代表着一种
对其他收缩机制的方便和有前景的探索
系统也是如此。
英文摘要
The long term goal of this research is to develop a better understanding of
the mechanisms underlying contractile processes and their regulation.
Contractility is an important property of many biological systems in
addition to that of skeletal muscle. The smooth muscles in the
gastro-intestinal tract provide the agitation necessary for proper
digestion and the smooth muscles in the circulatory system play a major
role. Contractile proteins in non-muscle cells are believed to be involved
in cell division, cytoplasmic streaming, phagocytosis and other processes
requiring modification and maintenance of cell shape. The contraction of
the platelet cell is important in the maturation of blood clots.
This project is directed along several major lines. The first involves
development of a better understanding of the skeletal muscle system. This
muscle is the best understood contractile system, but much remains to be
determined including the influence of the filamentous organization and the
detailed nature and control of the interaction between actin and myosin. A
second major line of research involves the application to other contractile
systems of the techniques developed with skeletal muscle. The smooth
muscle of chicken gizzard will be initially studied. Other possible
applications include the non-muscle contractile system of platelets. The
third major line of research is the use of the contractile proteins as a
model system for developing a general understanding of the influence on
enzymatic catalysis and regulation of having enzyme sites supramolecular
structures. This is a problem which is central to the extension of
enzymology from the study of reactions in dilute solutions in vitro into
the complexity of reactions as they occur in vivo.
Extensive use will be made of the exchange reaction of isotopically labeled
oxygen atoms between water and the phosphate produced by ATP hydrolysis.
Analysis of this exchange reaction has provided powerful insight into the
mechanism of skeletal myosin and continue to do so. It represents a
convenient and promising probe into the mechanisms of other contractile
systems as well.
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DOI:
--
发表时间:
1985
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
[Hackney,DD, Clark,PK]
通讯作者:
Clark,PK
Nonlinear dependence on actin of acto-heavy meromyosin 18O exchange and ATPase rate at low actin concentrations.
低肌动蛋白浓度下,重肌动球蛋白 18O 交换和 ATP 酶速率对肌动蛋白的非线性依赖性。
DOI:
--
发表时间:
1982
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
[Hackney,DD]
通讯作者:
Hackney,DD
A residence-time analysis of enzyme kinetics.
酶动力学的停留时间分析。
DOI:
10.1042/bj2430159
发表时间:
1987
期刊:
The Biochemical journal
影响因子:
--
作者:
[Sines,JJ, Hackney,DD]
通讯作者:
Hackney,DD
Analysis of positional isotope exchange in ATP by cleavage of the beta P-O gamma P bond. Demonstration of negligible positional isotope exchange by myosin.
通过 β P-O γ P 键的裂解分析 ATP 中的位置同位素交换。
DOI:
10.1021/bi00399a051
发表时间:
1987
期刊:
Biochemistry
影响因子:
2.9
作者:
[Dale,MP, Hackney,DD]
通讯作者:
Hackney,DD
Anomalous oxygen-18 exchange during ATP synthesis in oxidative phosphorylation.
氧化磷酸化中 ATP 合成过程中的异常氧 18 交换。
DOI:
10.1021/bi00368a047
发表时间:
1986
期刊:
Biochemistry
影响因子:
2.9
作者:
[Sines,JJ, Hackney,DD]
通讯作者:
Hackney,DD
共 6 条
REGULATION OF KINESIN MOTOR PROTEINS
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批准号:8322129
-
项目类别:
-
资助金额:$26.03万
-
财政年份:2010
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
REGULATION OF KINESIN MOTOR PROTEINS
-
批准号:8041386
-
项目类别:
-
资助金额:$27.52万
-
财政年份:2010
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
REGULATION OF KINESIN MOTOR PROTEINS
-
批准号:8134801
-
项目类别:
-
资助金额:$26.02万
-
财政年份:2010
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
REGULATION OF KINESIN MOTOR PROTEINS
-
批准号:8534289
-
项目类别:
-
资助金额:$25.13万
-
财政年份:2010
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
HOMOLOGIES OF MOTOR PROTEINS
-
批准号:6319765
-
项目类别:
-
资助金额:$0.13万
-
财政年份:1999
-
负责人:DAVID Daniel HACKNEY
-
依托单位:--
HOMOLOGIES OF MOTOR PROTEINS
-
批准号:6253558
-
项目类别:
-
资助金额:$0.61万
-
财政年份:1997
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:6490899
-
项目类别:
-
资助金额:$27.32万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:3415110
-
项目类别:
-
资助金额:$12.79万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:3415108
-
项目类别:
-
资助金额:$10.26万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:2267005
-
项目类别:
-
资助金额:$21.03万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:2488167
-
项目类别:
-
资助金额:$24.62万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:3415111
-
项目类别:
-
资助金额:$13.33万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:6070016
-
项目类别:
-
资助金额:$5.0万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:6343834
-
项目类别:
-
资助金额:$26.56万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:2267006
-
项目类别:
-
资助金额:$21.31万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:2267007
-
项目类别:
-
资助金额:$22.36万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:2858135
-
项目类别:
-
资助金额:$25.09万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:2037397
-
项目类别:
-
资助金额:$21.28万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM OF KINESIN ATPASE
-
批准号:6139488
-
项目类别:
-
资助金额:$25.81万
-
财政年份:1990
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
MECHANISM AND REGULATION OF CONTRACTILE PROTEINS
-
批准号:2078557
-
项目类别:
-
资助金额:$12.74万
-
财政年份:1979
-
负责人:DAVID Daniel HACKNEY
-
依托单位:
海外基金