GASTRIC MUCOSAL PERMEABILITY AND CYTOPROTECTION

胃粘膜通透性和细胞保护

• 批准号: 3151576
• 负责人: LAURENCE Y CHEUNG
• 金额: $ 8.7万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-07-01 至 1986-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3151576
• 关键词: acetylcholine; acid base balance; aspirin; atropine; bicarbonates; cimetidine; cyclic GMP; gastric juice inhibitor; gastric mucosa; gastrins; gastrointestinal agents; gastrointestinal circulation; gastrointestinal circulatory insufficiency; gastrointestinal pharmacology; histamine; microelectrodes; prostaglandin E; radioimmunoassay; radiotracer

Recently, gastric mucosa has been shown to actively secrete bicarbonate (HCO3-) into the lumen. This observation leads to a hypothesis that active secretion of HCO3- may be another important cytoprotective mechanism of the gastric mucosa, since the secreted HCO3-is likely to neutralize the intraluminal acid at the mucosal surface. To assess this hypothesis, the proposed studies are designed (1) to evaluate the role of HCO3- secretion on the acid-base status of the mucosa by measuring intramural pH and (2) to examine the changes in the rate of HCO3- secretion following various stimuli. They include (1) acetylsalicylic acid, a known gastric irritant, (2) Prostaglandin E2, a cytoprotective agent against experimental ulcerations, and (3) ischemia, a predisposing factor in acute ulcerogenesis. In addition, the following studies will determine if HCO3- secretion is affected by secretagogues such as acetylcholine, histamine and pentagastrin and/or mediated by cGMP. The experimental model for these studies will be an in vivo canine stomach which permits the calculation of HCO3- secretion by measuring intragastric pCO2 and pH as described by Garner and Flemstrom. This technique will be added to our present ability to measure gastric blood flow by gamma-labeled microspheres, mucosal cGMP by radioimmunoassay and intramural pH by the microelectrode technique.

最近，胃粘膜已被证明积极分泌碳酸氢盐（HCO 3-） 进入管腔。 这一观察结果导致了一种假设， HCO 3-可能是胃粘膜的另一重要细胞保护机制， 因为分泌的HCO 3-可能中和在细胞膜上的管腔内酸， 粘膜表面。 为了评估这一假设，拟议的研究设计 (1)为了评价HCO 3-分泌对胃肠道酸碱状态的作用， 通过测量壁内pH值来检查粘膜;（2）检查 HCO 3-各种刺激后的分泌。 它们包括（1）乙酰水杨酸 酸，一种已知的胃刺激物，（2）前列腺素E2，一种细胞保护剂 抗实验性溃疡，和（3）缺血，一个诱发因素， 急性溃疡形成 此外，以下研究将确定HCO 3- 分泌受促分泌素如乙酰胆碱、组胺和 五肽胃泌素和/或cGMP介导。 这些研究的实验模型 将是体内犬胃，允许计算HCO 3- 通过测量胃内pCO 2和pH分泌，如Garner所述， 弗莱姆斯壮 这项技术将被添加到我们目前的测量能力， 胃血流量的γ标记微球，粘膜cGMP的 放射免疫分析和微电极技术测定壁内pH值。

项目成果

Intracellular microelectrode studies of Necturus antral mucosa. Effect of aspirin on cell membrane potentials.

Necturus 窦粘膜的细胞内微电极研究。

• DOI: 10.1016/s0016-5085(85)80179-7
• 发表时间: 1985
• 期刊: Gastroenterology
• 影响因子: 29.4
• 作者: Cheung,LY;De,L;Ashley,SW
• 通讯作者: Ashley,SW

Microelectrode studies of Necturus antral mucosa. II. Equivalent circuit analysis.

Necturus 窦粘膜的微电极研究。

• DOI: 10.1152/ajpgi.1985.248.5.g574
• 发表时间: 1985
• 期刊: The American journal of physiology
• 作者: Ashley,SW;Soybel,DI;De,L;Cheung,LY
• 通讯作者: Cheung,LY

Measurement of regional gastric mucosal blood flow by hydrogen gas clearance.

通过氢气清除率测量局部胃粘膜血流量。

• DOI: 10.1016/0002-9610(84)90030-8
• 发表时间: 1984
• 期刊: American journal of surgery
• 影响因子: 3
• 作者: Cheung,LY;Sonnenschein,LA
• 通讯作者: Sonnenschein,LA

Comparison of gastric mucosal blood flow as measured by H2 gas clearance and microspheres during secretory stimulation and inhibition.

通过氢气清除率和微球在分泌刺激和抑制过程中测量的胃粘膜血流量的比较。

• 发表时间: 1985
• 期刊: Surgery
• 影响因子: 3.8
• 作者: Soybel,DI;Ashley,SW;Yan,ZY;Cheung,LY
• 通讯作者: Cheung,LY

Effect of cimetidine on canine gastric mucosal pH and blood flow.

西咪替丁对犬胃粘膜 pH 值和血流量的影响。

• DOI: 10.1016/0002-9610(83)90161-7
• 发表时间: 1983
• 期刊: American journal of surgery
• 影响因子: 3
• 作者: Cheung,LY;Sonnenschein,LA
• 通讯作者: Sonnenschein,LA