INSULIN AND CATECHOLAMINE IN CARBOHYDRATE METABOLISM

该项目旨在研究糖原磷酸化酶的调节。 在分离的大鼠肝细胞中由激素引起，特别是胰岛素和 阿尔法儿茶酚胺。阿尔法儿茶酚胺和加压素被认为是 随着细胞内钙的升高，继而激活磷酸化酶。我们的 人们的兴趣与α-肾上腺素能受体的性质有关，这种受体似乎 对于阿尔法的所有代谢影响，属于字母1而不是字母2类别 儿茶酚胺。我们感兴趣的是增加了的 添加磷脂酰肌醇后肝细胞内磷脂酰肌醇的转运 加压素、儿茶酚胺和胞浆钙升高。的确有 有证据表明，磷脂酰肌醇的周转率不会因进入而增加 细胞外钙离子。然而，这两个事件之间的联系仍然是 被建立起来。我们目前正在检验这样一种假设，即营业额的增加 磷脂酰肌醇参与了导致 细胞内结合钙的释放和细胞外钙的摄取。

The project is designed to investigate the regulation of glycogen phosphorylase in isolated rat hepatocytes by hormones with particular emphasis on insulin and alpha catecholamines. Alpha catecholamines and vasopressin are thought to activate phosphorylase secondary to an elevation of intracellular calcium. Our interests relate to the nature of the alpha-adrenergic receptors which appear to be of the alpha1 rather than alpha2 category for all metabolic effects of alpha catecholamines. We are interested in the relationship between the increased turnover of phosphatidylinositol seen in hepatocytes after the addition of vasopressin and catecholamines and the elevation of cytosol calcium. There is evidence that phosphatidylinositol turnover is not increased secondary to entry of extracellular calcium. However, the link between these two events remains to be established. We are currently testing the hypothesis that increased turnover of phosphatidylinositol is involved in the primary events which result in the release of bound intracellular calcium and the uptake of extracellular calcium.