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ACTIN BASED REGULATION OF SMOOTH MUSCLE CONTRACTION

基于肌动蛋白的平滑肌收缩调节

基本信息

批准号：
3153766
负责人：
JOSEPH M CHALOVICH
金额：
$ 8.06万
依托单位：
EAST CAROLINA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1985
资助国家：
美国
起止时间：
1985-04-01 至 1988-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3153766
关键词：
adenosine diphosphate adenosine triphosphate conformation muscle contraction muscle proteins phosphorylation smooth muscle stoichiometry stop flow technique vascular smooth muscle

项目摘要

The contraction of vertebrate smooth muscle, like all other types of muscle is the result of a cyclic interaction of the proteins myosin and actin in a process coupled to the hydrolysis of ATP. The regulation of smooth muscle contraction is very complex involving several distinct regulatory systems. Because of the great importance of the regulation of smooth muscle contraction, particularly in relation to vascular disease, the various components of these regulatory systems must be identified and the interactions which produce overall regulation must be defined. The primary regulatory system in smooth muscle appears to be myosin based with dephosphorylation of myosin causing relaxation. More evidence has been accumulating that an actin-linked system may further modulate the force producing interaction of myosin with actin. A likely candidate for an actin binding regulatory protein is the recently identified protein, caldesmon. The inhibitory function of caldesmon will be studied in detail. Its binding to actin and other proteins in the contractile apparatus will be quantitated. Finally, the interplay of myosin based and actin based regulatory systems in smooth muscle will be examined. Other potential regulatory proteins from smooth muscle may also be studied.

脊椎动物平滑肌的收缩，就像所有其他类型的肌肉一样，是蛋白质肌球蛋白和肌动蛋白在一个循环中相互作用的结果。这一过程与ATP的水解有关。平滑肌的调节收缩是非常复杂的，涉及几个不同的监管系统。由于平滑肌调节的重要性收缩，特别是与血管疾病有关，必须确定这些监管系统的组成部分，必须确定产生全面调节的相互作用。主平滑肌中的调节系统似乎是基于肌球蛋白的，肌球蛋白的去磷酸化引起松弛。更多的证据表明积累了肌动蛋白连接系统可以进一步调节力产生肌球蛋白与肌动蛋白的相互作用。一个可能的候选人，肌动蛋白结合调节蛋白是最近鉴定的蛋白质， caldesmon。钙调素的抑制功能将在详细它与肌动蛋白和其他蛋白质结合，仪器将被量化。最后，肌球蛋白的相互作用，将检查平滑肌中基于肌动蛋白的调节系统。其他也可以研究来自平滑肌的潜在调节蛋白。