CRYSTALLOGRAPHY OF ACTIN AND ACTIN-BINDING PROTEINS

肌动蛋白和肌动蛋白结合蛋白的晶体学

• 批准号: 3158198
• 负责人: CLARENCE E SCHUTT
• 金额: $ 13.13万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3158198
• 关键词: X ray crystallography; computer graphics /printing; conformation; electron density; intestinal villi; muscle contraction

It is proposed to determine the high resolution structure of the profilin:actin complex by x-ray crystallography, to establish the packing of the monomeric crystal structure in the helical actin filament, to crystallize villin and villin:actin complexes, and to determine the structures of a range of actin conformers in the profilin:actin crystals. A high resolution chemical structure of actin is essential for a mechanistic understanding of muscular contraction and the process of mechanochemical transduction. Very basic processes like cytokinesis, membrane ruffling, and endocytosis, common to many cells, all involve actin filaments and changes in the state of organization of actin-containing structures. The highly-conserved nature of actin and its widespread distribution in eukaryotic cells in processes where movement is involved, points to a common explanation at the chemical level of description. Knowledge of the actin structure, in both the monomeric and filamentous forms, will be important for unravelling the events involved when a cell, transformed by retroviruses, undergoes dramatic changes in shape. Similarly, during platelet activation, major re-organization of the cytoskeleton occurs. Actin-containing microvilli of the inner intestinal lumen medidate the absorptive process, and structures of these bundles may offer deeper insight into malabsorption diseases such as coeliac syndrome.

建议确定其高分辨率结构。 Profilin：肌动蛋白复合体经x射线结晶学测定，建立填充物 螺旋肌动蛋白细丝中的单体晶体结构， 结晶绒毛蛋白和绒毛蛋白：肌动蛋白复合体，并测定 肌动蛋白晶体中一系列肌动蛋白构象的结构。 肌动蛋白的高分辨化学结构对于 对肌肉收缩和肌肉收缩过程的力学理解 机械力化学转导。非常基本的过程，比如胞质分裂， 许多细胞常见的膜皱缩和内吞作用都与肌动蛋白有关。 含肌动蛋白的细丝和组织状态的变化 结构。肌动蛋白的高度保守性及其广泛存在 在涉及运动的过程中在真核细胞中的分布， 在描述的化学水平上指出了一个共同的解释。 了解肌动蛋白的单体和丝状结构 表格，将是重要的，当一个细胞， 被逆转录病毒转化，在形状上发生了戏剧性的变化。 类似地，在血小板激活期间，主要的重组 细胞骨架就出现了。内肠内含肌动蛋白的微绒毛 管腔是吸收过程的中间介质，并且这些束的结构可以 提供对吸收不良疾病的更深层次的洞察，如乳糜泻综合征。