TUMOR PROMOTOR AND CELL DIFFERENTIATION

肿瘤促进剂和细胞分化

• 批准号: 3166132
• 负责人: LEILA DIAMOND
• 金额: $ 11.95万
• 依托单位: WISTAR INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-09-01 至 1986-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3166132
• 关键词: carcinogenesis; cell differentiation; cell growth regulation; cell population study; cell type; chemical carcinogenesis; clone cells; cocarcinogen; environment related neoplasm /cancer; erythroid stem cell; gene expression; genetic transcription; genetic translation; human tissue; membrane activity; monoclonal antibody; mutagen testing; mutagens; neoplastic cell; phorbols; polyamines; radioimmunoassay; radiotracer; spectrometry; tumor promoters

Phorbol diester tumor promoters inhibit spontaneous and induced conversion of preadipose BALB/c 3T3 cells to adipocytes. This differentiation is also inhibited by the antiviral agent, interferon. To determine the mechanisms by which these compounds inhibit adipose conversion, and whether the mechanisms are the same or different, the effects of the inhibitors on various enzymes involved in glucose metabolism and fatty acid synthesis will be determined. The tumor promoters also induce differentiation in a line of human promyelocytic leukemia cells, HL-60. Variant HL-60 cells resistant to this effect of the promoters will be isolated in order to analyze the promoter-cell interactions that lead to the induction of differentiation in these cells. The studies on the effects of the promoters on blood cell differentiation will be extended to freshly isolated normal and leukemic bone marrow cells.

佛波醇二酯肿瘤促进剂抑制自发和诱导的 前脂肪BALB/c 3 T3细胞转化为脂肪细胞。 这种差异化也是 被抗病毒药物干扰素抑制。 确定机制， 这些化合物抑制脂肪转化，以及这些机制是否 相同或不同，抑制剂对各种相关酶的影响 在葡萄糖代谢和脂肪酸合成中的作用将被确定。 肿瘤 启动子还诱导人早幼粒细胞白血病细胞系的分化 细胞HL-60 对启动子的这种作用具有抗性的变体HL-60细胞 将被分离，以分析启动子-细胞相互作用，导致 诱导这些细胞的分化。 关于影响的研究 血细胞分化的启动子将扩展到新鲜分离的 正常和白血病骨髓细胞。