TERATOCARCINOMA AND EMBRYONAL TUMORS: SURFACE ANTIGENS

T/t复合物在细胞表面的特化中起作用。 似乎在控制细胞相互作用中重要的抗原， 在早期发展过程中认识到。 两项重要研究增加了 作为这种控制的模型的T/t复合体的功率：（1） 与致死突变之一（t）相关的抗原W18）已 在时间和地点上定位于遗传引起的功能障碍的部位 在突变胚胎;和（2）几个不同的T-致死基因已被 映射;他们是非等位基因和代表一个明显的基因家族传播 H-2位于17号染色体的中部，长度超过20 cM。 他们 我们打算研究t-突变染色质中的遗传相关基因 通过利用我们新发现的映射它们的能力：（1）通过经典的 （2）通过重组DNA技术。 我们的长期 目的是了解遗传控制的分子机制， 正常早期发育中的细胞定型和 胚胎性肿瘤 T/t复合体的致死基因揭示了一系列 野生型基因在控制早期 胚胎发育。 我们使用了经典孟德尔 遗传学和分子遗传学提供了一个详细的地图的t区 小鼠17号染色体 三个重要的发现已经出现：（1） 整个主要组织相容性复合体（MHC）在t-单倍型中是倒置的; (2)t-致死细胞排列成三个簇，其中最大的一个簇 围绕并与MHC混合;以及（3）不同的t基因， 分离高达15 cM，可以作为顺式/反式功能单元 试验表明，遗传可塑性可能在哺乳动物中发挥作用， 发展 （简体中文）

The T/t complex has a role in the specification of sets of cell surface antigens that appear to be important in controlling cell interactions and recognition during early development. Two important new studies increase the power of the T/t complex as a model of this control: (1) the antigen(s) associated with one of the lethal mutations (tw18) has been localized in time and place to the site of genetically caused dysfunction in the mutant embryo; and (2) several different t-lethal genes have been mapped; they are nonallelic and represent an apparent gene family spread over 20 cM of chromosome 17 with H-2 situated anomalously in the middle of them. We intend to study genetically relevant genes in t-mutant chromatin by taking advantage of our new-found ability to map them: (1) by classical breeding experiments; and (2) by recombinant DNA technology. Our long-term objective is to understand the molecular mechanisms and genetic control of cellular commitment in normal early development and noncommitment in embryonal tumors. The lethal genes of the T/t-complex reveal the existence of a series of wild-type genes that have an important role in the control of early embryonic development. We have used a combination of classical Mendelian genetics and molecular genetics to provide a detailed map of the t-region of mouse chromosome 17. Three important findings have emerged: (1) the entire major histocompatibility complex [MHC] is inverted in t-haplotypes; (2) the t-lethals are arranged in three clusters, the largest of which surrounds and is intermingled with the MHC; and (3) different t genes, separated by as much as 15 cM, can act as a functional unit in cis/trans tests implying that genetic plasticity may play a role in mammalian development. (CS)