MEROCYANINE DYES AS LEUKEMIA SPECIFIC PROBES

目的是使用独特的染料Mercury 540（MC 540）来研究 造血细胞膜的结构，以检查 白血病细胞和正常细胞之间的联系， 其在检测、诊断和治疗某些 白血病 Mercury 540（MC540）是一种荧光有机分子， 特别是白血病细胞的表面，从而使它们敏感， 到光解。 因为染料的特异性跨越了血统和 物种边界，它必须确定一些基本的所有功能 白血病细胞 事实上，人工脂质囊泡的结合研究 模型红细胞显示它能特异性地与细胞膜结合， 其中脂质被松散地包装。 在功能上，MC540结合膜 参与红系细胞中通过去核的受体消除， 巨噬细胞中的内吞作用，以及可能的受体消除或激活 在淋巴细胞中。 慢性粒细胞白血病患者红细胞 用MC540染色白血病（CML）提供了一个简单的系统， 这种分子缺陷不仅导致这些细胞染色， 也有白血病粒细胞，因为它们共同起源于一个缺陷的 干细胞，也可能证明是一个有用的标准，以遵循 在临床环境中的CML过程。 初步结果表明，MC540不仅可以识别红细胞， 来自CML患者的红细胞，也来自患有以下任何疾病的患者的红细胞： 我们测试的其它骨髓增生性疾病：真性红细胞增多症， 骨髓纤维化，伴髓样化生，原发性血小板增多症， 某些类型的急性髓细胞性白血病 因此， 红细胞MC540可作为一种简便的血液检测方法 骨髓增生性疾病的症状 （三）

The goals are to use the unique dye merocyanine 540 (MC540) to study the architecture of hematopoietic cell membranes, to examine the relationship between leukemia cells and their normal cell counterparts, and to explore its application in the detection, diagnosis, and treatment of certain leukemias. Merocyanine 540 (MC540) is a fluorescent organic molecule that binds specifically to the surfaces of leukemia cells and thereby sensitizes them to photolysis. Because specificity of the dye crosses both lineage and species boundaries, it must identify some feature fundamental to all leukemia cells. In fact, binding studies with artificial lipid vesicles and model erythrocytes have shown it to bind specifically to membranes in which the lipids are loosely packed. Functionally, MC540-binding membranes are involved in receptor elimination via enucleation in erythroid cells, endocytosis in macrophages, and possibly receptor elimination or activation in lymphocytes. The discovery that erythrocytes of patients with chronic myelogenous leukemia (CML) stain with MC540 offers a simple system in which to pursue the molecular defect responsible for staining of not only these cells, but also leukemic granulocytes because of their common origin in a defective stem cell, and may also prove a useful criterion with which to follow the course of CML in a clinical setting. Preliminary results indicate that MC540 can identify not only erythrocytes from patients with CML but also erythrocytes from patients with any of the other myeloproliferative diseases we have tested: polycythemia vera, myelofibrosis, with myeloid metaplasia, essential thrombocythemia and certain forms of acute myelogenous leukemia. Therefore, staining of erythrocytes with MC540 could provide a simple blood test for the detection of myeloproliferative diseases in general. (3)