TRANSFORMATION BY ABELSON MURINE LEUKEMIA VIRUS

阿贝尔森鼠白血病病毒的转化

• 批准号: 3167665
• 负责人: OWEN N. WITTE
• 金额: $ 14.3万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-05-01 至 1991-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3167665
• 关键词: Abelson leukemia virus; Retroviridae disease; disease /disorder model; gene expression; genetic manipulation; genetic regulation; helper virus; myelogenous leukemia; oncogenic virus; radiotracer; serology /serodiagnosis; surface antigens; temperature sensitive mutant; tissue /cell culture; viral leukemogenesis; virus RNA; virus genetics

This proposal will investigate the molecular mechanisms used by the abl oncogene in its normal and abnormal growth regulation. Comparison of the structures, regulation, hematopoietic transformation activities, and tyrosine kinase enzymatic properties of the murine cellular, and viral forms of the abl gene as well as the translocation derived form of the abl gene found in Chronic Myelogenous Leukemia cells will be performed. Emphasis will be placed on establishing transformed cell models for murine stem cell populations under the regulation of abl or other oncogenes in concert with improved culture techniques. These studies should aid our understanding of the mechanisms of oncogenesis and hematopoietic development.

这项建议将调查的分子机制所使用的abl 癌基因在正常和异常生长调节中的作用。 比较 结构、调节、造血转化活性，以及 鼠细胞和病毒的酪氨酸激酶的酶性质 ABL基因的形式以及ABL的易位衍生形式 将进行慢性粒细胞白血病细胞中发现的基因。 重点将放在建立小鼠转化细胞模型， 在ABL或其他癌基因调控下的干细胞群体， 配合改良的栽培技术。 这些研究应该有助于我们 了解肿瘤发生和造血机制 发展