SELF-RENEWAL IN NORMAL/LEUKEMIC HEMOPOIETIC STEM CELLS

正常/白血病造血干细胞的自我更新

• 批准号: 3167122
• 负责人: DONALD METCALF
• 金额: $ 3.09万
• 依托单位: WALTER AND ELIZA HALL INST MEDICAL RES
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-06-01 至 1986-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3167122
• 关键词: cell differentiation; cell growth regulation; cellular oncology; chemical related neoplasm /cancer; clone cells; embryo /fetus tissue /cell culture; erythropoietin; granulocyte; hematopoietic stem cells; leukopoietic factor; macrophage; mixed tissue /cell culture; myelogenous leukemia; neoplasm /cancer transplantation; viral leukemia

Purification studies on medium conditioned by pokeweed mitogen-stimulated spleen lymphocytes (SCM) have shown that the active factor able to stimulate stem cell self-renewal is the glycoprotein Multi-CSF (IL-3). An inducible T-lymphocyte cell line (LB3) has been developed that synthesizes large amounts of Multi-CSF. The cDNA for Multi-CSF has been cloned from an mRNA library constructed from LB3 cells. The action of cloned Multi-CSF will be analyzed on the self-renewal of stem cells in three cell systems--normal marrow stem cells, Multi-CSF-dependent continuous multipotential cell lines, and v-ras-transformed hemopoietic cells. Studies will be continued on the mechanism of action of G-CSF in suppressing stem cell self-renewal in the myelomonocytic leukemia WEHI-3B, and attempts will be made to clone the receptor for G-CSF on these cells.

商陆有丝分裂原刺激条件培养液的纯化研究 脾淋巴细胞(SCM)的活性因子已显示出能够 刺激干细胞自我更新的是糖蛋白多粒细胞集落刺激因子(IL-3)。一个 可诱导的T淋巴细胞系(LB3)已经建立起来，它可以合成 大量的多种脑脊液。多粒细胞集落刺激因子基因的克隆 从LB3细胞构建的mRNA文库。克隆人的行为 多粒细胞集落刺激因子对三种细胞中干细胞自我更新的影响 系统--正常骨髓干细胞，多种脑脊液依赖的连续细胞 多潜能细胞系和v-ras转化的造血细胞。 将继续研究G-CSF的作用机制。 抑制粒单核细胞白血病WEHI-3B的干细胞自我更新 并将尝试克隆这些细胞上的G-CSF受体。