PHOTORADIATION THERAPY OF INDUCED DYSPLASIA

某些有机化学物质和可见光的结合产生了一种 细胞毒作用可能是通过形成单氧。 血卟啉衍生物（HPD）是目前研究最深入的化合物 产生这种“光动力效应”。 暴露于较短波长的 比与细胞毒性相关的光更轻的光引起HPD发荧光。 HPD 比正常组织更大程度地保留在肿瘤组织中 在全身给药后。 由于这种特性， 光动力效应可局限于肿瘤病变， 周围的正常组织 这种选择性的细胞毒性已经被 在几种类型的动物和人类肿瘤中得到证实，但尚未被证实。 在上皮内瘤变中报告。 这种恶性肿瘤的光辐射 病变不会受到这种类型的主要限制的影响， 治疗，光的组织穿透性差。 由于这些病变是 通常小于4 mm的深度散射和光能损失不是一个好的选择。 重要因素。 上皮内瘤形成的动物模型已经被 用氨基甲酸乙酯和十四烷酰-佛波醇-乙酸酯诱导 (TPA)在BDF 1小鼠的皮肤上。 这些动物将接受HPD， 暴露于来自氩离子泵浦染料激光器的630 nm波长的光。 将研究异型增生病变的选择性光破坏 组织学上 从动物研究中收集的信息将用于 设计一项人体试验 希望这种方法容易实施， 基本上无毒的治疗形式最终可以应用于 人类外阴、阴道和子宫颈的癌前病变。 在这些位置的上皮内瘤变的光辐射将是 比目前使用的手术方式更好。

The combination of certain organic chemicals and visible light produces a cytotoxic effect probably through the formation of single oxygen. Hematoporphyrin derivative (HPD) is the most thoroughly studied compound producing this "photodynamic effect". Exposure to shorter wavelengths of light than that associated with cytotoxicity causes HPD to fluoresce. HPD is retained in neoplastic tissue to a greater extent than normal tissue following systemic administration. Because of this property the photodynamic effect can be limited to neoplastic lesions with sparing of surrounding normal tissue. This selective cytotoxicity has been demonstrated in several types of animal and human tumors but has not been reported in intraepithelial neoplasia. Photo-radiation of this malignant lesion would not be affected by the main limitation of this type of therapy, poor tissue penetration of light. Since these lesions are typically less than 4 mm in depth scatter and loss of light energy is not a significant factor. An animal model of intraepithelial neoplasia has been developed by induction with urethane and tetradecanoyl-phorbal-acetate (TPA) in the skin of BDF1 mice. These animals will be given HPD and exposed to light of 630 nm wavelength from an argon ion pumped dye laser. Selective photodestruction of dysplastic lesions will be studied histologically. Information collected from animal studies will be used in designing a pilot human study. It is hoped that this easily administered, essentially non-toxic form of therapy can eventually be applied to pre-malignant lesions of the vulva, vagina, and cervix in humans. Photoradiation of intraepithelial neoplasia in these locations would be preferable to currently used surgical modalities.