INCIDENCE, QUANTITATION OF A TUMOR PROTEIN (ONCOMODULIN)

肿瘤转化改变了几种肿瘤的基础浓度 钙结合蛋白，钙调蛋白，S-100，和皮肤钙结合蛋白。这个 癌发育钙结合蛋白癌调节蛋白也可能出现在 肿瘤，但与其他肿瘤不同的是，它不在 正常啮齿动物或人类成年组织，并出现在85%的肿瘤中 不同的来源。它是长期目标，以适应的外观 在钙转运和钙离子转运中的这一普遍扰动 调节蛋白，它必须对细胞内的某些部分负责 肿瘤表型。我们必须确定是否表达了 癌调节蛋白基因与特定癌基因的功能有关，或 将病毒携带的癌基因插入细胞的特定部分 基因组，或者说是对肿瘤发展的一般反应。也可以是 令人感兴趣的是通过DNA从化学上是否转染正常细胞 转化细胞在诱发肿瘤的同时激活肿瘤调节蛋白基因 转型。这将通过数量和质量两方面来实现 侵染多种不同细胞类型的肿瘤素的筛选 转化缺陷和温度敏感型致癌病毒 突变，或被不同的化学致癌物转化。蜂窝手机 寻找肿瘤素的功能将通过建立首个超 该蛋白的结构亚细胞定位，并通过确定 肿瘤性调节蛋白负责肿瘤的性质，如侵袭性，通过 用正常侵袭的滋养层细胞作为模型，这是 制造这种蛋白质的少数非肿瘤细胞。

Neoplastic transformation changes the basal concentration of several calcium-binding proteins, calmodulin, S-100, and skin CaBP. The oncodevelopmental calcium-binding protein oncomodulin may also appear in neoplasia, but is different from these others in that it is not found in normal rodent or human adult tissue, and appears in 85% of tumours of diverse origin. It is the long term objective to fit the appearance of oncomodulin into this general perturbation in calcium transport and modulator proteins which must be responsible for some part of the neoplastic phenotype. We must determine whether the expression of the oncomodulin gene is related to the functioning of a particular oncogene, or insertion of a virally borne oncongene into a specific part of the cell genome, or is a general response to neoplastic development. Also of interest is whether transfection of normal cells by DNA from chemically transformed cells activates the oncomodulin gene while inducing neoplastic transformation. This will be achieved by both quantitative and qualitative oncomodulin screening of many different cell types infected by competent, transformation-defective, and temperature sensitive oncogenic viral mutants, or transformed by different chemical carcinogens. The cellular function of oncomodulin will be sought by establishing first the ultra- structural subcellular location of this protein, and by determining whether oncomodulin is responsible for tumour properties, such as invasiveness, by using as a model the normally invasive trophoblast cell, which is one of the few non-neoplastic cells that make this protein.