FC-GAMMA RECEPTOR-MEDIATED REGULATION OF MACROPHAGE

这项工作的总体目标是阐明分子机制。 触发的细胞间和细胞内通信的 免疫球蛋白G与Fc-γ受体(Fc-Gamma-R)的特异性结合 存在于B细胞、巨噬细胞等细胞表面。这个 具体目标是：(1)确定初始生化的性质 Fc-γ-Rs在小鼠巨噬细胞表面传递的信号和 巨噬细胞系和(2)研究其生化序列 FC-FC-GAMMA-R发送的初始信号之后的事件 互动。这些目标将通过以下方式实现：(1)孤立和 Fc-γ-Rs，磷脂酶A2，腺苷环化酶， 和cAMP依赖的蛋白激酶以及(2)Fc-γ-R触发的研究 环氧合酶和脂氧合酶途径、cAMP合成和激活 在细胞水平上的cAMP依赖的蛋白激酶。所获得的结果 有助于理解生物化学的基础 在免疫反应中发挥重要作用的细胞相互作用 将军。(CS)

The overall objective of the work is to elucidate the molecular mechanism of cell-cell and intracellular communications that are triggered by the specific binding of the immunoglobulin G to Fc-gamma receptors (Fc-gamma-R) present on the surface of B cells, macrophages, and other cell types. The specific aims are to: (1) determine the nature of an initial biochemical signal transmitted by Fc-gamma-Rs on the surface of murine macrophages and macrophage-cell lines and (2) investigate the biochemical sequence of events following the initial signal transmitted by the Fc-Fc-gamma-R interaction. These goals will be approached by: (1) isolation and characterization of Fc-gamma-Rs, phospholipase A2, adenylate cyclases, and cAMP-dependent protein kinases and (2) studies of Fc-gamma-R-triggered cyclooxygenase and lipooxygenase pathways, cAMP synthesis, and activation of cAMP-dependent protein kinases at the cell level. The results obtained should contribute to the understanding of the biochemical basis for the cellular interactions that play important roles in the immune response in general. (CS)