HUMAN LYMPHOCYTES CLONALLY TRANSFORMED BY EBV

人类淋巴细胞的精确个体发育范围易受 EB病毒（EBV）的转化尚未定义。 最近 但不完全的证据表明，EBV可能会改变一系列的 B淋巴细胞表型，从“早期”（非Ig产生）B细胞到 前B细胞和B细胞。 IG表达和细胞遗传学分析 Burkitt肿瘤细胞中的现象表明， 将它们与其他EBV转化细胞区分开来可能与 这是免疫球蛋白基因分化过程中的一个特殊阶段 这 致癌事件可包括人c-myc基因易位。 我们将 试图更好地定义人类淋巴细胞的个体发生范围 易受EBV介导的转化。 原代单核细胞来自 将克隆转化人胎儿、新生儿和成人组织， 琼脂糖培养基。 然后将克隆繁殖的品系通过以下方法进行表型分型： 分析IG表达、表面“Ia”抗原和其它表面 表位 还将进行核型分析。 然后细胞克隆 分析其IG基因重排模式。 细胞克隆 将检查未表达的IG基因等位基因的异常重排 细胞c-myc基因重排的证据，或染色体 易位 我们的研究结果应该能让我们更清楚地了解 人类（B-）淋巴细胞个体发育的各个阶段， EB病毒转化 我们也许能部分地阐明 临床恶性B细胞克隆出现从多克隆转化 人口

The precise ontogenetic range of human lymphocytes susceptible to transformation by Epstein-Barr Virus (EBV) has not been defined. Recent but incomplete evidence suggests that EBV may transform a range of B-lymphocyte phenotypes, from "early" (non-Ig producing) B-cells through pre-B cells and B-cells. Analyses of Ig expression and cytogenetic phenomena in Burkitt tumor cells suggest that the oncogenic event which distinguishes them from other EBV-transformed cells may be associated with a particular stage in their Ig-gene differentiation program. This oncogenic event may include a human c-myc gene translocation. We will attempt to better define the ontogenetic range of human lymphocytes susceptible to EBV-mediated transformation. Primary mononuclear cells from human fetal, neonatal, and adult tissues will be clonally transformed in agarose medium. Clonally propagated lines will then be phenotyped by analysis of Ig expression, surface "Ia" antigen, and other surface epitopes. Karyotype analyses will also be done. The cell clones will then be analyzed for their patterns of Ig gene rearrangements. Cell clones with aberrant rearrangements of non-expressed Ig gene alleles will be examined for evidence of rearrangement of the cellular c-myc gene, or chromosomal translocation. The results of our studies should yield a clearer picture of the stages of human (B-) lymphocyte ontogeny which are susceptible to transformation by EBV. We may be able to partially illuminate how clinically malignant B-cell clones emerge from a polyclonally transformed population.