SV40-INDUCED CHANGES IN GROWTH REGULATION IN HOST CELLS

本研究的目的是了解机制（S） S期特异性基因在正常和SV 40- 被感染的细胞 由于这些基因的表达与 随着细胞的生长状态，希望了解 它们的调节将有助于理解 正常细胞和病毒感染细胞的生长。 这 理解这一点对研究致癌基因也很重要。 转换，因为转换的细胞的属性之一是 他们无法正常调节自己的生长 研究S相调节的方法 特异性基因将是分子基因，使用胸苷激酶 (TK)基因作为模型系统。 TK表达在 细胞周期，G 0和G1期细胞较低， 细胞进入S期。 基因组和cDNA TK克隆 将用于构建一系列突变和杂交TK基因。 然后将这些改变的基因转染到组织培养中 细胞，并将检查它们的表达的调节。 利用这些方法， 监管将被解决：1）在什么水平的监管， 基因发生（转录/转录后等）？ （二） 什么样的TK DNA序列负责这种调节？ 第三章 病毒蛋白在诱导TK基因中的作用是什么？ 四、 什么样的细胞因子参与诱导TK 吉恩？

The objective of this research is to understand the mechanism(s) by which S-phase specific genes are regulated in normal and SV40- infected cells. Since the expression of these genes is correlated with the growth state of the cell, it is hoped that an understand of their regulation will contribute to an understanding of the control of cell growth in normal and virus-infected cells. This understanding will in turn be important to the study of oncogenic transformation, since one of the properties of transformed cells is that they fail to regulate their growth in a normal way. The approach taken to investigating the regulation of S-phase specific genes will be a molecular one, using the thymidine kinase (TK) gene as a model system. TK expression is regulated during the cell cycle, being low in G0 and G1 phase cells, and increasing as the cells enter S phase. Both genomic and cDNA TK clones will be used to construct a series of mutant and hybrid TK genes. These altered genes will then be transfected into tissue culture cells, and the regulation of their expression will be examined. Using these techniques, the following questions concerning TK regulation will be addressed: 1) At what levels does regulation of the gene occur (transcriptional/post-transcriptional, etc.)? 2) What TK DNA sequences are responsible for this regulation? 3) What is the role of viral proteins in inducing the TK gene? 4) What cellular factor or factors are involved in inducing the TK gene?