MONOCLONAL ANTIBODIES IN CLASSIFICATION OF TUMORS

单克隆抗体在肿瘤分类中的应用

• 批准号: 3174973
• 负责人: HECTOR A. BATTIFORA
• 金额: $ 12.47万
• 依托单位: CITY OF HOPE NATIONAL MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3174973
• 关键词: cancer registry /resource; diagnosis quality /standard; electron microscopy; histochemistry /cytochemistry; histopathology; human tissue; immunoelectron microscopy; immunofluorescence technique; monoclonal antibody; neoplasm /cancer classification /staging; neoplasm /cancer genetics; neoplasm /cancer immunodiagnosis; neoplasm /cancer relapse /recurrence; prognosis; tissue /cell preparation; tumor antigens

The purpose of this research project is to evaluate monoclonal antibodies capable of permitting increased diagnostic accuracy in histopathology or of identifying significant prognostic groups. Monoclonal antibodies obtained from several sources will be screened against a panel of normal human tissues and selected neoplasms of variable histogenesis and degrees of differentiation. A bank of frozen tumors maintained at -80~C at the Division of Anatomic Pathology, City of Hope will be the primary source of material. In addition, paraffin-embedded tumors fixed in absolute alcohol, buffered formalin, and other fixatives will also be tested, if warranted by preliminary study, and compared with the results obtained from frozen sections. Transmission electron microscopy and electron microscopy immunochemistry will be done on selected cases of diagnostic difficulty. Various immunohistochemical methods will be initially employed on frozen and paraffin-embedded tissues fixed in various fixatives to maximize selection of the optimal handling for each antigen/antibody. Antibodies which after this screening show tissue or tumor discriminatory capacity will be tested against a larger group of well-characterized neoplasms of appropriate type depending on the specificity shown by prior tests. The results of the immunohistochemical studies will be correlated with the histopathologic diagnosis, tumor grade, stage of disease, response to therapy, survival, and other prognostic parameters of specific significance for the neoplasms involved. (2)

本研究项目的目的是评估单克隆抗体 能够提高组织病理学诊断的准确性， 识别显著的预后组。 获得的单克隆抗体 将对一组正常人进行筛选， 不同组织发生和程度的组织和选定肿瘤 分化 冷冻肿瘤库维持在-80 ℃， 希望之城解剖病理学分部将成为 材料 此外，石蜡包埋的肿瘤在无水乙醇中固定， 缓冲福尔马林和其他固定剂也将进行检测，如果有必要， 初步研究，并与冷冻 路段 透射电镜和电子显微镜 将对诊断困难的选定病例进行免疫化学检查。 各种免疫组织化学方法将最初采用冷冻 石蜡包埋的组织固定在各种固定剂中， 选择每种抗原/抗体的最佳处理。 抗体 其在该筛选后显示出组织或肿瘤辨别能力 将针对更大一组特征良好的肿瘤进行测试， 根据先前测试显示的特异性确定合适的类型。 的 免疫组织化学研究的结果将与 组织病理学诊断、肿瘤分级、疾病分期、对 治疗、生存和其他具有特定意义的预后参数 对于肿瘤的治疗 （二）