MN2+ -PHOSPHOLIPID STIM PK AND LEUKEMIC DIFFERENTIATION
MN2 -磷脂刺激 PK 和白血病分化
基本信息
- 批准号:3183949
- 负责人:
- 金额:$ 15.45万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1986
- 资助国家:美国
- 起止时间:1986-05-01 至 1994-11-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This project deals with the biochemistry and cellular physiology of a newly
identified protein kinase, referred to as "PK-P", for
phospholipid-modulated protein kinase, and its major endogenous substrate
pp73. This system is detectable in murine and human leukemic cells,
wherein it may play a role in multi-colony stimulating factor (IL-3) signal
transduction and chemically inducible differentiation. pp73 has been
purified, and will be characterized further, with respect to the
proteolytic activity which it has been observed to exhibit; its interaction
with phospholipids and divalent cations, and with protein kinases as a
substrate; and its structure as determined by partial amino acid sequencing
and immunologic analysis. PK-P has also been found to readily
phosphorylate ribosomal protein S6. This reaction will be further
quantitated and compared to phosphorylation by other kinases, and its
effects upon in vitro translation determined. Precursors, other subunits
or interacting activities, or modifications modulating PK-P will be
identified. The pattern of response of the PK-P/pp73 system to chemical
and biologic differentiation inducing agents of the HL-60 human myeloid
leukemic cell line, and to IL-3 treatment of the DA-1 murine leukemic cell
line will be further characterized using direct measurement of protein and
phosphorylation levels and subcellular distribution by protein
immunoblotting techniques. Interaction of these components with membranes
from such cells subjected to various such treatments will also be studied.
Molecular biologic studies aimed towards the cloning of the cDNA gene for
PK-P will be undertaken, so that the complete primary structure of PK-P can
ultimately be determined.
这个项目涉及一种新的生物化学和细胞生理学
鉴定的蛋白激酶,称为"PK-P",用于
磷脂调节蛋白激酶及其主要内源性底物
第73页。 该系统在鼠和人白血病细胞中是可检测的,
其中它可能在多集落刺激因子(IL-3)信号传导中起作用
转导和化学诱导分化。 pp73已经
纯化,并将进一步表征,关于
已观察到其表现出的蛋白水解活性;其相互作用
与磷脂和二价阳离子,并与蛋白激酶作为
底物;以及通过部分氨基酸测序确定的其结构
和免疫分析。 PK-P也被发现很容易
磷酸化核糖体蛋白S6。 这种反应将进一步
定量并与其他激酶的磷酸化进行比较,
确定对体外翻译的影响。 前体,其他亚单位
或相互作用的活动,或修饰调节PK-P将是
鉴定 PK-P/pp73系统对化学物质的反应模式
和HL-60人髓系的生物分化诱导剂,
白血病细胞系,以及IL-3对DA-1鼠白血病细胞的治疗
将使用蛋白质的直接测量来进一步表征细胞系,
蛋白质磷酸化水平和亚细胞分布
免疫印迹技术。 这些组分与膜的相互作用
还将研究来自经受各种这样的处理的这样的细胞的细胞。
分子生物学研究的目的是克隆的cDNA基因,
将进行PK-P,以便PK-P的完整一级结构可以
最终要确定。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
LAURENCE ELIAS其他文献
LAURENCE ELIAS的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('LAURENCE ELIAS', 18)}}的其他基金
MN2+ -PHOSPHOLIPID STIM PK AND LEUKEMIC DIFFERENTIATION
MN2 -磷脂刺激 PK 和白血病分化
- 批准号:
3183958 - 财政年份:1986
- 资助金额:
$ 15.45万 - 项目类别:
MN+2-PHOSPHOLIPID STIM PK AND LEUKEMIC DIFFERENTIATION
MN 2-磷脂刺激 PK 和白血病分化
- 批准号:
3183954 - 财政年份:1986
- 资助金额:
$ 15.45万 - 项目类别:
MN++/PHOSPHOLIPID-STIMULATED PROTEIN KINASE & LEUKEMIA
MN /磷脂刺激的蛋白激酶
- 批准号:
2090787 - 财政年份:1986
- 资助金额:
$ 15.45万 - 项目类别:
MN+2-PHOSPHOLIPID STIM PK AND LEUKEMIC DIFFERENTIATION
MN 2-磷脂刺激 PK 和白血病分化
- 批准号:
3183950 - 财政年份:1986
- 资助金额:
$ 15.45万 - 项目类别:
MN+2-PHOSPHOLIPID STIM PK AND LEUKEMIC DIFFERENTIATION
MN 2-磷脂刺激 PK 和白血病分化
- 批准号:
3183947 - 财政年份:1986
- 资助金额:
$ 15.45万 - 项目类别:
MN2+ -PHOSPHOLIPID STIM PK AND LEUKEMIC DIFFERENTIATION
MN2 -磷脂刺激 PK 和白血病分化
- 批准号:
3183953 - 财政年份:1986
- 资助金额:
$ 15.45万 - 项目类别:
MN++/PHOSPHOLIPID-STIMULATED PROTEIN KINASE & LEUKEMIA
MN /磷脂刺激的蛋白激酶
- 批准号:
2090788 - 财政年份:1986
- 资助金额:
$ 15.45万 - 项目类别:
MN+2-PHOSPHOLIPID STIM PK AND LEUKEMIC DIFFERENTIATION
MN 2-磷脂刺激 PK 和白血病分化
- 批准号:
3183955 - 财政年份:1986
- 资助金额:
$ 15.45万 - 项目类别:
MN2+ -PHOSPHOLIPID STIM PK AND LEUKEMIC DIFFERENTIATION
MN2 -磷脂刺激 PK 和白血病分化
- 批准号:
3183952 - 财政年份:1986
- 资助金额:
$ 15.45万 - 项目类别:
MN2+ -PHOSPHOLIPID STIM PK AND LEUKEMIC DIFFERENTIATION
MN2 -磷脂刺激 PK 和白血病分化
- 批准号:
3183957 - 财政年份:1986
- 资助金额:
$ 15.45万 - 项目类别:
相似海外基金
Hedgehog signalling in T-cell differentiation and function
T 细胞分化和功能中的 Hedgehog 信号传导
- 批准号:
BB/Y003454/1 - 财政年份:2024
- 资助金额:
$ 15.45万 - 项目类别:
Research Grant
Comparative single-cell analysis of disease-derived stem cells to identify the cell fate defect on the cell differentiation trajectory
对疾病来源的干细胞进行比较单细胞分析,以确定细胞分化轨迹上的细胞命运缺陷
- 批准号:
23H02466 - 财政年份:2023
- 资助金额:
$ 15.45万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The role of cell differentiation in colorectal cancer progression
细胞分化在结直肠癌进展中的作用
- 批准号:
23K06661 - 财政年份:2023
- 资助金额:
$ 15.45万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Dissecting the role of hypoxia in T cell differentiation in cancer
剖析缺氧在癌症 T 细胞分化中的作用
- 批准号:
10578000 - 财政年份:2023
- 资助金额:
$ 15.45万 - 项目类别:
Mechanisms mediating human enteroendocrine cell differentiation and function
介导人肠内分泌细胞分化和功能的机制
- 批准号:
10739834 - 财政年份:2023
- 资助金额:
$ 15.45万 - 项目类别:
TOX-driven CD8 T cell differentiation and dysfunction in tumors
TOX驱动的肿瘤中CD8 T细胞分化和功能障碍
- 批准号:
10586679 - 财政年份:2023
- 资助金额:
$ 15.45万 - 项目类别:
Elucidation of molecular mechanisms of immune cell differentiation of a novel Rab protein in hematopoietic stem cells
阐明造血干细胞中新型Rab蛋白免疫细胞分化的分子机制
- 批准号:
23K16122 - 财政年份:2023
- 资助金额:
$ 15.45万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
New strategies in cell replacement therapies for diabetes: role of USP7 in iPSC and adult organoids beta cell differentiation
糖尿病细胞替代疗法的新策略:USP7 在 iPSC 和成体类器官 β 细胞分化中的作用
- 批准号:
MR/X01813X/1 - 财政年份:2023
- 资助金额:
$ 15.45万 - 项目类别:
Research Grant
Role of alveolar fibroblasts in extracellular matrix organization and alveolar type 1 cell differentiation
肺泡成纤维细胞在细胞外基质组织和肺泡1型细胞分化中的作用
- 批准号:
10731854 - 财政年份:2023
- 资助金额:
$ 15.45万 - 项目类别:
Exhaustive Identification of Essential Genes for Human Taste Cell Differentiation ~Development of a Method for Inducing Differentiation of Taste Buds from ES/iPS Cells~
彻底鉴定人类味觉细胞分化必需基因~开发诱导ES/iPS细胞味蕾分化的方法~
- 批准号:
23K09214 - 财政年份:2023
- 资助金额:
$ 15.45万 - 项目类别:
Grant-in-Aid for Scientific Research (C)