SEQUENTIAL 1H NMR STUDIES FOLLOWING CHEMOTHERAPY

这些研究的目的是调查和记录1H核磁共振 动物和动物化疗和激素治疗后的变化 人类肿瘤，并调查导致 这些变化。基于我们之前对这两个项目进行的核磁共振研究 人类和动物肿瘤模型，这些核磁共振变化可能被证明是有用的 快速评估特定治疗方案的有效性，如下 对正常组织的治疗毒理学，以及在设计 基于核磁共振生物学变化的时序治疗。这个 研究将包括使用大型肿瘤肿块模型和 微转移肝脏模型，均为M5076肿瘤。肿瘤核磁共振的研究 体内和体外将与总的研究相关 水分含量、胞外水分、总脂和细胞动力学 测量。肿瘤生长和发生过程中的变化 将分析处理后的T1、T2和自旋密度的变化。 连续的人体体内核磁共振研究肝转移，骨转移， 化疗后的T1和其他核磁共振参数的测量 激素治疗将与临床和实验室相关 变化的证据。描述类似变化的适当动物模型 将被用来确定核磁共振变化的生物原因， 为将这些参数用于非侵入性监测做准备 并用于设计治疗方案。

The purpose of these studies is to investigate and document the 1H NMR changes that occur after chemotherapy and hormonal therapy in animal and human tumors, and to investigate the biologic changes that contribute to these alterations. Based on prior NMR studies, performed by us, on both human and animal tumor models, these NMR changes may prove to be useful in rapidly assessing the efficacy of a particular treatment program, following the toxicology of treatment on normal tissues, and in designing time-sequential treatments based on the biology of NMR changes. The studies will include work with a large tumor mass model and a micrometactatic liver model, both of the M5076 tumor. Studies of tumor NMR in-vivo and in-vitro will be correlated with simultaneous studies of total water content, extracellular water, total lipids and cytokinetic measurements. Changes occuring during tumor growth and occuring during treatment will be analyzed, along with T1, T2 and spin density NMR changes. Sequential human in-vivo NMR studies of liver metastases, bone metastases, with measurements of T1 and other NMR parameters, following chemotherapy and hormonal therapy will be correlated with clinical and laboratory evidence of changes. Appropriate animal models describing similar changes will be used to identify the biologic reasons for the NMR changes, in preparation for using these parameters for non-invasive monitoring of patients and for designing treatment programs.