ANTICARCINOGENIC AGENTS IN ORANGE PEEL OIL

橙皮油中的抗癌剂

• 批准号: 3176161
• 负责人: MICHAEL N GOULD
• 金额: $ 2.88万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3176161
• 关键词: antineoplastics; benzanthracenes; breast neoplasms; cancer prevention; carcinogenesis; carcinogenesis inhibitor; chemical carcinogenesis; drug /agent; drug metabolism; fruit; high performance liquid chromatography; histopathology; human tissue; laboratory mouse; laboratory rat; mass spectrometry; neoplasm /cancer nutrition therapy; nuclear magnetic resonance spectroscopy; nutrition related tag; radiation carcinogenesis

The research proposed here seeks to define the efficacy of the use of orange peel oil as an anticarcinogen while further defining its mechanism of action in this role. To accomplish this HPLC techniques will be used to isolate the components of orange peel oil. The anticarcinogenic potency of these components will be quantitated by feeding them in a totally defined diet to rats exposed to DMBA. Carcinogen specificity will be defined by testing the ability of the orange peel oil anticarcinogen(s) to inhibit both spontaneous and induced mammary tumors. Organ specificity will be examined by testing the ability of the orange peel oil anticarcinogen(s) to inhibit neoplastic changes in mammary gland, skin and bladder. Species specificity studies will compare the response of carcinogen exposed rats and mice to orange oil anticarcinogens. In addition, similar in vitro comparisons will be made using rat and human primary parenchymal cells from mammary gland and liver. Individual specificity will be modeled with rat strains possessing high or low sensitivity to the induction of mammary carcinomas. All animal experiments will be analyzed using both parametric and nonparametric statistical procedures. Once the anticarcinogen is identified its pharmacokinetics and metabolism will be defined. The biological activity of these metabolites will be determined. The mechanism(s) of action of the orange peel oil anticarcinogen(s) and/or its metabolites will be studied.

这里提出的研究旨在确定使用 橙子皮油作为抗癌剂，同时进一步确定其机制 在这个角色上的行动。 为了实现这一点，将使用HPLC技术来 分离出橙子皮油的成分。 的抗癌效力 这些组分将通过将它们送入完全定义的 给暴露于DMBA的大鼠喂食。 致癌物特异性定义如下： 测试所述橙子皮油抗癌剂抑制 自发性和诱发性乳腺肿瘤。 器官特异性将 通过测试橙子皮油抗癌剂的能力来检查， 抑制乳腺、皮肤和膀胱的肿瘤变化。 物种 特异性研究将比较致癌物暴露大鼠的反应， 和小鼠对橙子油抗癌剂的耐受性。 此外，类似的体外 将使用大鼠和人的原代实质细胞进行比较， 乳腺和肝脏。 个体特异性将采用大鼠模型进行建模 对乳腺癌诱导具有高或低敏感性的菌株 癌 所有动物实验都将使用两个参数进行分析 和非参数统计程序。 一旦抗癌药 将确定其药代动力学和代谢。 的 将测定这些代谢物的生物活性。 的 橙子皮油抗癌剂和/或其 将研究代谢物。