NEOPLASTIC AND NORMAL T CELL DIFFERENTIATION ANTIGENS

这项研究的目的是获得基本的结构，遗传， 一种新的小鼠T细胞分化抗原的生物学信息 （BM 100）。 使用单克隆抗体筛查正常和 肿瘤细胞表明，BM 100是T细胞特异性的，除了它是 在大量的骨髓样本中发现 根据这一发现， 似乎BM 100存在于前T细胞上。 这一假设将是 通过包括流式细胞术在内的技术组合进行检查， 免疫印迹和骨髓嵌合体。 结构信息 使用免疫印迹，凝集素柱，放射性标记， 以及一维和二维凝胶分析。 编码BM 100的cDNA 使用cDNA表达载体从T细胞肿瘤中分离抗原 λ gt 11。 这些克隆将被测序并用于遗传作图 问题研究 它们还将用于分离和表征基因组 克隆 BM 100很有趣，因为它可能代表了最早的 T细胞的标志物。BM 100也很有趣，因为它可能 代表人抗原（gpL 115）的小鼠同源物。 gpL115 最近发现，在患有 Wiskott-Aldrich综合征，一种X连锁免疫缺陷疾病， 在其他缺陷中，有T细胞异常，通常会导致 淋巴网状恶性肿瘤

The purpose of this research is to obtain basic structural, genetic, and biological information on a novel mouse T cell differentiation antigen (BM100). Initial findings using a monoclonal antibody to screen normal and neoplastic cells suggest that BM100 is T cell specific except that it is found on a high pecentage of bone marrow cels. Based on this finding it appears that BM100 is present on pre-T cells. This hypothesis will be examined by a combination of techniques including flow cytometry, immunoblots, and bone-marrow chimeras. Structural information on the protein will be obtained using immunoblots, lectin columns, radiolabelling, and one and two dimensional gel analysis. The cDNAs encoding the BM100 antigen will be isolated from a T cell tumor using a cDNA exprssion vector lambda gt11. These clones will be sequenced and used in genetic mapping studies. They will also be used to isolate and characterize genomic clones. The BM100 is interesting since it may represent the earliest marker of T lineage cells. The BM100 is also interesting since it may represent the mouse homolog of a human antigen (gpL115). The gpL115 molecule has recently been found to be altered in patients with Wiskott-Aldrich Syndrome, an X-linked immunodeficiency disorder, which among other defects has T cell abnormalities and often leads to lymphoreticular malignancies.