Using SBEM and cellular electron tomography to study the basal body/pro-basal body linker.

使用 SBEM 和细胞电子断层扫描来研究基体/前基体连接体。

• 批准号: BB/M000532/1
• 负责人: Sue Vaughan
• 金额: $ 60.17万
• 依托单位: Oxford Brookes University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM000532%2F1
• 关键词: Using; SBEM; cellular; electron; tomography

Nearly all cells in the human body contain an organelle called a centriole or basal body which has multiple functions. It is important in organising the mitotic spindle that segregates chromosomes when cells divide. It assembles a structure called a cilium or flagellum that allow cells such as sperm cells to move and cilia are found in cells in the lining of the lungs where they aid the movement of liquids. Cilia are the antenna of cells in Humans to sense the external cellular environment. Most organisms contain cilia or flagella and they are important for locomotion in many diverse single celled organisms as well as allowing cells to attach to surfaces. There is a great deal of interest in basal bodies because defects in the assembly of flagella and cilia and defects in duplication of basal bodies have been implicated in a range of human diseases that are collectively called the ciliopathies. These diseases include polycystic kidney disease, retinal degeneration, Kartagener's syndrome, Bardet-Biedl syndrome. These latter two diseases, for example are associated with a broad spectrum of pathologies, such as chronic bronchitis, male sterility, obesity and diabetes.This bewildering array of pathologies is due, in part, to the many functions of basal bodies in the human body. Parasitic organisms such as Trypanosoma brucei which causes African sleeping sickness and Nagana in cattle, Trypanosoma cruzi, which causes Chagas disease in South America, as well as the malaria parasite Plasmodium all rely on the assembly and function of a flagellum and is a major virulence factor that is being investigated by researchers.Basal bodies are small organelles in cells and electron microscopy is necessary to show us the structural complexity of this organelle. There have been recent developments in three dimensional microscopy which helps biologists to understand many aspects of the spatial organisation of cells. Two new techniques called serial block face scanning electron microscopy and array tomography are allowing biologists, for the first time, to reconstruct three dimensional views of individual cells and large areas of tissue such as synapse connections in the brain. Part of this grant to to work with these new techniques and develop ways in which we can gather and analyse large data sets.

人体中几乎所有的细胞都含有一种称为中心粒或基体的细胞器，它具有多种功能。它在组织细胞分裂时分离染色体的有丝分裂纺锤体中很重要。它组装了一种称为纤毛或鞭毛的结构，允许精子细胞等细胞移动，纤毛存在于肺内层的细胞中，它们有助于液体的移动。纤毛是人体细胞的触角，用于感知外部细胞环境。大多数生物体含有纤毛或鞭毛，它们对于许多不同的单细胞生物体的运动以及允许细胞附着在表面上都很重要。由于鞭毛和纤毛的组装缺陷以及基底体的复制缺陷与一系列统称为纤毛病的人类疾病有关，因此对基底体有很大的兴趣。这些疾病包括多囊肾病、视网膜变性、Kartagener综合征、Bardet-Biedl综合征。例如，后两种疾病与广泛的病理学有关，如慢性支气管炎、男性不育症、肥胖症和糖尿病，这些令人困惑的病理学部分是由于人体中基底体的许多功能。寄生生物，如引起非洲昏睡病和牛的Nagana的布氏锥虫，引起南美恰加斯病的克氏锥虫，以及疟原虫都依赖于鞭毛的组装和功能，是研究人员正在研究的主要毒力因子。基体是细胞中的小细胞器，电子显微镜是必要的，以显示我们的结构复杂性这个细胞器。最近三维显微镜的发展有助于生物学家了解细胞空间组织的许多方面。两种新技术称为连续块面扫描电子显微镜和阵列断层摄影术，首次允许生物学家重建单个细胞和大面积组织的三维视图，如大脑中的突触连接。这笔赠款的一部分将用于使用这些新技术，并开发收集和分析大型数据集的方法。

项目成果

CEP164C regulates flagellum length in stable flagella.

• DOI: 10.1083/jcb.202001160
• 发表时间: 2021-01-04
• 期刊: The Journal of cell biology
• 作者: Atkins M;Týč J;Shafiq S;Ahmed M;Bertiaux E;De Castro Neto AL;Sunter J;Bastin P;Dean SD;Vaughan S
• 通讯作者: Vaughan S

Molecular model of the mitochondrial genome segregation machinery in Trypanosoma brucei.

• DOI: 10.1073/pnas.1716582115
• 发表时间: 2018-02-20
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Hoffmann A;Käser S;Jakob M;Amodeo S;Peitsch C;Týč J;Vaughan S;Zuber B;Schneider A;Ochsenreiter T
• 通讯作者: Ochsenreiter T

A key regulatory protein for flagellum length control in stable flagella

稳定鞭毛中鞭毛长度控制的关键调节蛋白

• DOI: 10.1101/872952
• 发表时间: 2019
• 作者: Atkins M

Scanning and three-dimensional electron microscopy methods for the study of Trypanosoma brucei and Leishmania mexicana flagella.

• DOI: 10.1016/bs.mcb.2014.12.011
• 发表时间: 2015
• 期刊: METHODS IN CELL BIOLOGY
• 作者: Gluenz, Eva;Wheeler, Richard John;Hughes, Louise;Vaughan, Sue
• 通讯作者: Vaughan, Sue

Proteomic identification of novel cytoskeletal proteins associated with TbPLK, an essential regulator of cell morphogenesis in Trypanosoma brucei.

• DOI: 10.1091/mbc.e15-04-0219
• 发表时间: 2015-09-01
• 期刊: Molecular biology of the cell
• 影响因子: 3.3
• 作者: McAllaster MR;Ikeda KN;Lozano-Núñez A;Anrather D;Unterwurzacher V;Gossenreiter T;Perry JA;Crickley R;Mercadante CJ;Vaughan S;de Graffenried CL
• 通讯作者: de Graffenried CL